BcMag™ Magnetic Beads for Protein Purification
Biomagnetic separation technique has been widely used in biological sciences. The technique has the advantage of rapid, simple, and flexible handling of large or microliter scale biological samples without the need for laborious repeated pipetting and centrifuging. Amerigo Scientific offers a broad product portfolio of unique and high-quality superparamagnetic beads including affinity magnetic beads and reversed-phase magnetic beads for fractionation of proteins, peptides, cells, bacteria, antibodies, DNA/RNA and other molecules from complex biological samples. These functional magnetic beads can be compatible with commercially available automatic magnetic processor or with simple bench top manual separators.
BcMag™ magnetic beads are uniform, monodispersed, spherical, core-shell superparamagnetic beads. The bead consists of a nanometer-scale superparamagnetic iron oxide core completely encapsulated by a high purity silica shell.
Features and Advantages of BcMag™ Magnetic Beads
- Fast (60% ion content)
- Very low non-specific binding
- Organic solvent resistant
- High binding capacity
- Broad range of working temperature
- Easy sterilization and autoclavable
Specifications of BcMag™ Magnetic Beads
| Composition |
Silica-coated iron oxide |
| Bead Size (diameter) |
~1 µm (cv ≤ 5%); ~5 µm (cv ≤ 10%) |
| Number of Beads (beads/mg) |
~1.7 x 10⁸ (1µm); ~5 x 10⁶ (5 µm) |
| Surface Area |
>100 m²/g |
| Magnetization |
~40 - 45 EMU/g |
| Type of Magnetization |
Superparamagnetic |
| Effective Density |
2.5 g/ml |
| Stability (pH) |
4.0-10 (long-term); 3< >11 (short-term) |
| Stability (temperature) |
4 °C – 140 °C |
| Stability (organic solvents) |
Most organic solvents |
BcMag™ Ion Exchange Magnetic Beads
Amerigo Scientific offers a wide range of BcMag™ ion exchange magnetic beads that are uniform, silica-coated superparamagnetic beads for quick fractionation of proteins or peptides from complex biological samples such as serum and plasma manually or automatically.
A charged protein or peptide binds to an oppositely charged matrix. BcMag™ WAX magnetic beads or BcMag™ SAX magnetic beads should be selected if the isoelectric point (pI) of the target protein or peptide is below the pH of the buffer. If the pI of the target protein or peptide is above the pH of the buffer, BcMag™ WCX magnetic beads or BcMag™ SCX magnetic beads should be selected.
Product Range
BcMag™ Affinity Magnetic Beads
Magnetic affinity chromatography is a unique separation tool in biochemistry, clinical chemistry, environmental testing, and pharmaceutical science. It has been widely used for quick and efficient purification and analysis of compounds from complex biological samples with minimal nonspecific adsorption.
Amerigo Scientific offers a variety of affinity magnetic beads with reactive functional groups that can be used to covalently conjugate almost any custom ligands. Short- and long- arm magnetic beads are available.
Available Functional Groups
Affinity Purification Steps
The interaction between a ligand and its complementary macromolecule depends significantly on the length of a linker, since a suitable linker length provides an optimal distance from the bead surface. Long-arm magnetic beads can be used to couple low molecular weight ligands, while both short- and long- arm magnetic beads can be used for conjugation of high molecular weight ligands. In addition, coupling conditions including pH, temperature, and buffer may influence the ligand stability and coupling efficiency. For different applications, the selection of appropriate magnetic bead type is crucial for the success of affinity purification.
Product Range
Amerigo Scientific also offers conjugation buffer kits for different BcMag™ magnetic Beads. A kit contains conjugation buffer, blocking buffer, wash buffer, and coupling reagent.
BcMag™ Reversed-phase Beads
Amerigo Scientific offers BcMag™ reversed-phase magnetic beads for quickly purifying, desalting and concentrating femtomolar to picomolar scale of peptides or proteins from biological fluids and other aqueous sample manually or automatically. BcMag™ reversed-phase magnetic beads are uniform, silica-based superparamagnetic beads with high density of hydrophobic groups on the surface.
Product Range
| Product Name |
Description |
|
BcMag™ C-18 Magnetic Beads
|
Beads containing hydrophobic C18 alkyl groups on their surface that can be used for purification, desalting and concentration of low molecular weight proteins or peptides |
| BcMag™ C-4 Magnetic Beads
|
Beads containing hydrophobic C4 alkyl groups on the surface that can be used for purification, desalting and concentration of large molecular weight proteins and peptides |
| BcMag™ C-8 Magnetic Beads
|
Beads containing hydrophobic C8 alkyl groups on the surface that can be used for low to intermediate molecular weight proteins, which are bound too strongly retained on C-18 beads |
| BcMag™ Cyanopropyl magnetic beads
|
Beads containing cyanopropyl groups on the surface that can be used for very hydrophobic peptides or proteins which may be irreversibly retained on more hydrophobic C-18 beads |
| BcMag™ Diphenyl magnetic beads
|
Beads containing hydrophobic diphenyl groups on the surface that can be used for quickly purifying femtomolar to picomolar scale of polypeptides with aromatic side chains, large hydrophobic proteins, membrane-spanning peptides, and lipid peptides |
| BcMag™ Phenyl magnetic beads
|
Similar in polarity to C-8 beads; some unique selectivity and retention produced by electron dense aromatic ring |
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