Two-dimensional (2D) cell culture in a plastic flasks or plates is easy, low time-consuming, low-cost technic and effortless to interpret. However, monolayer cell culture does not reflect the growth environment of cells in vivo. Three-dimensional (3D) cell culture is an innovative cell culture method that aims to overcome the limitations of 2D cell culture and will be refined, promising to replace expensive animal models for in vivo studies.

LifeGel for 3D cell culture is a ready-to-use plate containing protein-base hydrogel. Cells seeded on the surface of LifeGel can form 3D structures, like spheroids. All LifeGel products are available in 48-well and/or 96-well plate format with different standard cell media, including RPMI, DMEM-HG (Standard), DMEM-LG, DMEM/F-12, alpha-MEM, or PBS.

Characteristics of LifeGel

  • Protein-based
  • Batch to batch repeatable
  • Defined hydrogel stiffness
  • Easily digestible
  • Customizable for different cells and assays
  • Supply for small and large users
  • Compatible with standard imaging platforms
  • Growth factor free

Product Range

Product Name Description
LifeGel for 3D cell culture 48- or 96-well plate containing standard hydrogel
Panel of LifeGels for 3D cell culture 48-well plate with a whole range of types of LifeGel
Panel of 16 different hydrogels in triplicate or panel of 24 different hydrogels in duplicate
LifeGel Digestion Kit The product allows the quick and gentle release of single cells grown on LifeGel while preserving the highest-possible yield.
-  Sufficient for digestion of a 48-well plate
Tissue Digestion Kit The product allows the quick and gentle release of single cells from primary tissue while preserving the highest-possible yield.
-  Sufficient for digestion of a 16 g tissue
LifeGel for spheroidal growth of MSC 96-well plate covered by the LifeGel in the presence of a cell culture medium or PBS
-  For spheroidal growth of mesenchymal stem cells
LifeGel for cancer spheroid formation Multi-well plate covered by the LifeGel in the presence of a cell culture medium or PBS
-  For cancer spheroid formation
LifeGel Starter Pack Two plates (48- or 96-well plates) containing LifeGel for 3D cell culture or Panel of LifeGels for 3D cell culture. Digestion kit is included.

Applications

Industrial Applications

  • Drug screening (using cell line, PDXs or patient samples)
  • LifeGel microcarriers for suspension cell culture (digestible microcarriers)
  • Advanced research models for hit confirmation studies
  • Intestinal permeability assay

Basic Research Applications

  • Cancer biology
  • Tumor formation
  • Cell migration
  • injectable for tumor growth
  • 3D cell migration test
  • Other applications

Cell Cultured on LifeGel

Primary cells HUVEC - human umbilical vein endothelial cells (for angiogenesis research)
PDX - patient derived xenograft
Human cell lines LoVo - colorectal adenocarcinoma
LS180 - colorectal adenocarcinoma
U-2 0S- bone osteosarcoma
PANC-1- pancreatic cancer
MDA-MB-231-breast cancer
Immortalized HUVEC (for angiogenesis research)
Murine cell lines Pan_02 - pancreatic ductal adenocarcinoma
4T1- breast cancer
B16-F10 -skin; melanoma
L929- fibroblast
Hamster cell lines Pan_02- pancreatic ductal adenocarcinoma
4T1-breast cancer
B16-F10-skin; melanoma
L929- fibroblast

Example Applications

Staining of porcine mesenchymal stem cells (pMSCs) growing on LifeGel (α-Tubulin – red, F-actin- green, nuclei – blue). Images on different heights, performed with objective 10X.Staining of porcine mesenchymal stem cells (pMSCs) growing on LifeGel (α-Tubulin – red, F-actin- green, nuclei – blue). Images on different heights, performed with objective 10X.

Cell Lines Cultured on LifeGelCell Lines Cultured on LifeGel

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