Reverse phase chromatography (RPC) resins are important alternatives to silica-based RPC media because of their have unique selectivity, high capacity and wide pH stability range. Amerigo Scientific offers 3 types of polymeric RPC bulk media, UniPS®, UniPMN, and UniPMM. All of them are monodispersed, rigid divinylbenzene or methacrylic copolymer beads using proprietary precision microsphere technologies, designed for high-resolution and high-capacity purification of a wide range of substances, from natural products, peptides, oligonucleotides to large proteins.

UniPS®, UniPSN, and UniPMM resins have been mainly applied in preparative RPC purification of peptides, oligonucleotides, proteins, and natural products, while UniPS® products of small particle sizes (especially non-porous resins) have been used as analytical chromatography media. These high-performance chromatography media can also be used as reverse phase solid phase extraction (SPE) sorbents. Because of their superior pH stability and unique selectivity, these polymeric RPC products are especially suitable for purification with high pH requirements.

Characteristics

Characteristics UniPS® UniPSN UniPMM
Support Matrix Poly(styrene/ divinylbenzene) Poly(N-Vinyl Pyrrolidone /divinylbenzene) Polymethacrylate
Surface Polarity Low Medium High
Surface Functionality None, native polymer matrix
Bead Form Monodisperse, spherical, rigid
Particle Size (µm) 3, 5, 10, 15, 20, 30, 40 30, 60 40
Chemical Stability Can be used with all aqueous and organic solvents commonly used in reverse phase chromatography
Working pH Range 1 to 14 2 to 13 2 to 12
CIP pH Range 1 to 14 1 to 14 1 to 14
Maximum Pressure Drop < 10 MPa < 4 MPa < 1.6 MPa
Operating Temperature 4 to 40 °C 4 to 40 °C 4 to 40 °C
Delivery Conditions 20% ethanol suspension or dry powder    

  • Unique Selectivity

Conventional organic polymeric RPC matrix, such as poly(styrene-divinylbenzene), can provide different selection from that of silica-based RPC media, especially in the separation of biomolecules. In addition to high performance polystyrene (styrene-diethylbenzene) media known as UniPS®, Amerigo Scientific offers a range of polymer matrix with increased polarity, including UniPSN and UniPMM. The higher polarity makes them different from conventional polystyrene-divinylbenzene and become another option of especial separation selectivity to remove the impurity.

  • High Column Efficiency and High Capacity

Due to the uniform size and perfectly spherical shape nature of UniPS®, UniPSN and UniPMM reverse phase media, they generally provide better column efficiency and better RPC resolution than conventional polymer RPC media. And the pore size distribution of UniPS®, UniPSN and UniPMM is optimized so that they have high load capacity.

  • Excellent Chemical and pH Stability

UniPS®, UniPSN, and UniPMM RPC media exhibit excellent chemical and pH stability. pH stability of UniPS® media is especially superior because they are based on poly(styrene-co-divinylbenzene) with inherently unreactive property and the entire pH range of 1 to 14. The chemical and pH stability gives the polymeric RPC media unparalleled flexibility in operating conditions and cleaning procedures, as well as superior lifetime.

  • Quality Assurance

The quality control examination of each batch of RPC media products includes measurement of particle size, size distribution, pore size and surface area, as well as performance tests of model protein retention time, resolution, loading capacity, etc.

Products

Product Name Cat. No. Particle Diameter Pore Size Size
UniPS® 3-300 02000-003030 3 μm 300 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 5-100 02000-005010 5 μm 100 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 5-300 02000-005030 5 μm 300 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 5-500 02000-005050 5 μm 500 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 5-1000 02000-005100 5 μm 1000 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 10-100 02000-010010 10 μm 100 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 10-300 02000-010030 10 μm 300 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 10-500 02000-010050 10 μm 500 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 10-1000 02000-010100 10 μm 1000 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 15-100 02000-015010 15 μm 100 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 15-300 02000-015030 15 μm 300 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 15-500 02000-015050 15 μm 500 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 15-750 02000-015075 15 μm 750 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 15-1000 02000-015100 15 μm 1000 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 20-100 02000-020010 20 μm 100 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 20-300 02000-020030 20 μm 300 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 20-500 02000-020050 20 μm 500 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 20-1000 02000-020100 20 μm 1000 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 30-100 02000-025030 30 μm 100 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 30-300 02000-030030 30 μm 300 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 30-800 02000-030050 30 μm 800 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 30-1000 02000-030100 30 μm 1000 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 40-100 02000-040010 40 μm 100 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 40-300 02000-040030 40 μm 300 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 40-500 02000-040050 40 μm 500 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 40-800 02000-040080 40 μm 800 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 40-1000 02000-040100 40 μm 1000 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPS® 50-300 02000-050030 50 μm 300 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPSA 30-300 02000-230030 30 μm 300 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPMM 40-500 02000-440050 40 μm 500 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPMM 50-Carb 02006-450030 50 μm   30ml; 100ml; 500ml; 1L; 10L; 100L
UniPSN 30-300 02000-330030 30 μm 300 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPSN 40-300 02000-340030 40 μm 300 Å 30ml; 100ml; 500ml; 1L; 10L; 100L
UniPSN 60-300 02000-360030 60 μm 300 Å 30ml; 100ml; 500ml; 1L; 10L; 100L

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