• Amerigo Scientific Instrument

    Expression systems utilizing mammalian cells can perform appropriate protein folding, complex N-linked glycosylation, authentic O-linked glycosylation, and extensive post-translational modifications, which are important for complete biological activity of proteins. The gene expression in mammalian cells requires a suitable cell line and appropriate vectors. These vectors introduce specific genes into mammalian cells, transcribe those genes, and subsequently produce modified proteins. Mammalian expression vector systems promote constitutive and inducible expression of post-translational modified and appropriately folded proteins. In addition, these vectors can characterize the impact of specific mutations on cell metabolism, and stably alter cellular phenotype as a function of transgene expression.

    Mammalian expression vectors are generally divided into two categories, namely viral vectors and plasmid vectors. Viral vectors are essentially inactivated viruses into which genes are cloned, such as adenoviruses, retroviruses, and lentiviruses. Plasmid vectors are more complex, and their functional components include prokaryotic, eukaryotic, and viral sequences. Prokaryotic elements facilitate bacterial propagation and vector maintenance, and eukaryotic and viral elements comprise transcription elements and sequence coding for selectable markers. Transcriptional regulation involves the coordinated interaction of multiple genetic elements. The regulation of gene expression in a plasmid or viral vector is controlled by promoter and enhancer sequences as well as signal sequences required for efficient processing of transcripts. The most common promoters and enhancers are of viral origin. Locus control regions (LCRs) can be used in specific cell types to regulate gene expression. In addition to transcriptional control elements and translational control elements, mammalian expression vectors contain selectable markers for vector propagation in bacteria, as well as markers for selection of transfected cells, preparation of stable cell lines, and gene amplification.

    When designing a DNA transfer and expression study, the primary factors to consider in selecting expression vectors are the type of promoter/enhancer sequence, transient or stable expression type, and the desired protein expression amount. Amerigo Scientific offers powerful vectors for high-level transient or stable expression in a wide range of mammalian cells.

    Products (146)
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    TBS7201
    • Size: 20 uL
    • Catalog Number: MEV1505489TRI
    TBS7202
    • Size: 20 uL
    • Catalog Number: MEV1505488TRI
    TBS7305
    • Size: 20 uL
    • Catalog Number: MEV1505487TRI
    TBS7304
    • Size: 20 uL
    • Catalog Number: MEV1505486TRI
    TBS7303
    • Size: 20 uL
    • Catalog Number: MEV1505485TRI
    TBS7301
    • Size: 20 uL
    • Catalog Number: MEV1505484TRI
    TBS7302
    • Size: 20 uL
    • Catalog Number: MEV1505483TRI
    TBS7306
    • Size: 20 uL
    • Catalog Number: MEV1505482TRI
    TBS7401
    • Size: 20 uL
    • Catalog Number: MEV1505481TRI
    TBS7102
    • Size: 20 uL
    • Catalog Number: MEV1505480TRI
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