In general, the term immobilization refers to the act of the limiting movement or making incapable of movement. Immobilization is a universal phenomenon existing in nature. In the fields of biological and biomedical, many molecules such as biopolymers and small molecules are artificially immobilized on solid support as important research tools. The solid support should exhibit good chemical and physical stability and contain functional groups that can be bound to the active molecule. Common solid supports are magnetic particles, agarose beads, nanoparticles, films, microplates, array surfaces, and strips. The main methods for immobilization are adsorption, covalent binding, entrapment, encapsulation, and crosslinking, and the appropriate method should be selected according to the research purpose. The formation of covalent bonds between the ligand and the support usually provides the stronger linkages compared with other immobilization methods. The types of functionalities used for ligand attachment are easily reactive components such as amines, sulfhydryls, aldehydes, and carboxylic acids.
Product Range
Amerigo Scientific offers SepSphere™ kits for the immobilization of small molecules and large biomolecules (including proteins, antibodies, peptides, and oligonucleotides) on agarose beads. All processes of immobilization, washing of unreacted starting materials, and column packing are performed in a column. The required consumables and reagents are available in the SepSphere™ kit. The washing is performed using a simple manual push syringe without the need for gas/air flow or vacuum.
Features of SepSphere™ Immobilization Kits
- All reagents, buffers, and consumables for immobilization included in the kits
- Agarose beads with high loading capacity and stability
- Pre-made buffers for easy direct use
- Immobilization, washing, and packing all performed within a column
- Minimum requirement for immobilization set-up
Design of Columns in SepSphere™ Immobilization Kits
The column in the SepSphere™ kit is the core component of molecular immobilization. The column consists of five parts.
- Male luer lock cap for facilitating fluid mixing
- Column top and luer attachment. The luer attachment is used as a tight seal and attachment of the syringe for washing. The column top can be removed to allow buffer addition and agitation.
- Column body containing a polypropylene frit at the bottom
- Additional frit for column packing after immobilization
- Bottom female luer lock plug for facilitating fluid mixing and storage
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