Solid-phase peptide synthesis (SPPS) is the method of choice for peptide preparation in both research and industrial, which greatly simplifies the removal of reagents and solvents. The principle of SPPS is based on attaching the first amino acid to a resin, then proceeding with peptide chain successive elongation to ultimately synthesize the target peptide. The SPPS method has the advantages of easy purification, short synthesis cycle, and easy automation. Many long-chain peptides, cyclic peptides, and challenging peptides have been synthesized using SPPS. N-α-Boc protected amino acid building blocks (Boc/Benzyl strategy) were used. Subsequently, the Fmoc/t Bu strategy gained popularity mainly because it avoided the use of hazardous hydrogen fluoride (HF) for cleavage from the solid support and final deprotection.
SPPS resin is composed of a polymeric solid support linked permanently to a linker (also called bifunctional spacer or handle) that facilitates temporary anchoring of the first amino acid to the polymeric solid support. Polystyrene is the most common core resin in SPPS, and other core matrices include polyacrylate, polyacrylamide, and polyethylene glycol. Chloromethylated polystyrene is the most fundamental substituted polystyrene resin, which is commonly called Merrifield resin. Aminomethyl (AM) resin that linked to various linkers through a stable amide bond has long been used in SPPS as a core resin. The core resins themselves are of limited use as peptide synthesis resins, and their cleavage properties need to be modified by attaching suitable linkers. There are a wide variety of linkers for polystyrene core resins, such as Wang and Rink. Wang linkers produce COOH products, and Rink linkers produce amide products.
SPPS resins have good swelling in most organic solvents and good swelling degree is beneficial to the reaction yield. In addition, the average size of polymeric beads has an influence on the reaction time. The larger the bead, the slower the diffusion, resulting in a longer reaction time. Amerigo Scientific offers a wide variety of peptide synthetic resins with complete specifications and stable performance.
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