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Overview
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BcMag™ Iodoacetyl-activated Magnetic Beads are uniform, silica-based superparamagnetic beads coated with high density Iodoacetyl functional groups on the surface. The beads are used to covalently conjugate sulfhydryl (-SH)-containing ligands. BcMag™ Iodoacetyl-activated Magnetic Beads are most suitable for conjugation of large proteins. BcMag™ Long-arm Iodoacetyl-activated Magnetic Beads are recommended for conjugation of small peptides because the long-arm (20-atom) hydrophilic linker may reduce steric hindrance.
• Covalently and specifically couples with cysteine residues
• Water-insoluble ligands can be conjugated in coupling buffer containing final concentration of 10-30% acetonitrile, or dimethylformamide (DMF), or dimethylsulfoxide (DMSO), or of 6 M Guanidine•HCl.
• Stable covalent bond with minimal ligand leakage
• Produces reusable immunoaffinity matrices
• Low nonspecific binding
• Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads
• Applications: Purification for Antibodies, Proteins/Peptides, DNA/RNA; Cell sorting, ImmunoprecipitationPlease contact us at for specific academic pricing.
More Details
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- Properties
- Applications
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Overview