agarose

Electrophoresis Gels

The separation matrixes of nucleic acid electrophoresis include agar, agarose, polyacrylamide, and composite agarose-acrylamide gels. Among them, agarose and polyacrylamide are the two most used gels in nucleic acid electrophoresis. Polyacrylamide gels are suitable for separating nucleic acid molecules smaller than 1000 bp. More porous agarose gels can be used to separate mixtures of larger fragments up to 20kb.

The concentration of the gel determines the pore size of the gel which affects the migration of nucleic acids. The resolution of nucleic acids varies with the percentage concentration of the gel. Increasing the agarose concentration of the gel reduces the migration rate and improves the separation of smaller nucleic acid molecules, while decreasing the concentration of the gel allows the separation of larger nucleic acid molecules.

Agarose

For agarose gel electrophoresis, 0.7% gel shows good separation or resolution for 5-10 kb DNA fragments, while 2% gel shows good resolution for 0.2-1 kb DNA fragments. Up to 3% can be used to separate very tiny fragments, but high concentration gels require longer running time and are tend to bebrittle and may not set uniformly.

Amerigo Scientific offers agarose powders and convenient tablets for size-based separation of nucleic acids in electrophoresis application.

To streamline the workflow and save time, convenient agarose  tablets are supplied.To streamline the workflow and save time, convenient agarose tablets are supplied.

Benefits

  • DNA fragments are quickly separated with a consistent resolution.
  • Low EEO property enables high electrophoretic mobility of DNA.
  • Strong gel strength allows easy handling and less breakage.
  • No detectable DNase and RNase activities.

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