BcMag™ Quick DNA/RNA Purification kits
Purification of target nucleic acids is an important step before many biotechnological processes such as detection, cloning, sequencing, amplification, hybridization, cDNA synthesis, etc., none of which can be performed with crude sample materials. The presence of a large number of cellular or other contaminating materials in complex sample mixtures often impedes many subsequent reactions and techniques. Thus, many applications that rely on DNA or RNA identification require effective, reliable, and reproducible methods to isolate nucleic acids from complex mixtures. In contrast to other techniques, magnetic separation of nucleic acids can directly isolate nucleic acids from crude materials such as blood, tissue homogenates, culture media, and water. Since the magnetic properties of solid materials can be adjusted, they can be selectively removed relatively easily from viscous sample suspensions. In addition, the efficiency of magnetic separation is particularly suitable for large-scale purification.
Magnetic carriers with immobilized affinity ligands or prepared from biopolymers that exhibit affinity to the target nucleic acid are used in the separation process. Many magnetic carriers are magnetic particles made from different synthetic polymers, biopolymers, porous glass, or magnetic particles based on inorganic magnetic materials. Paramagnetic particles, especially suitable materials, do not interact with each other in the absence of a magnetic field. These particles are magnetized in strong magnetic fields, but do not retain permanent magnetism once the field is gone. In the process of reaction, magnetic aggregation and agglomeration of particles are prevented, which ensures easy suspension of particles and uniform nucleic acid extraction.
BcMag™ Quick DNA/RNA Purification Kits
Amerigo Scientific offers a variety of DNA/RNA purification kits using proprietary BcMag™ magnetic beads, designed for quick purification of high quality of DNA or RNA from different types of samples. BcMag™ purification kits are magnetic beads-based kits that do not require laborious repeat pipetting and centrifugation. Kit components include BcMag™ magnetic beads, lysis /binding buffer, washing buffer, and elution buffer.
Features and Advantages
- Fast, easy, one-step high-throughput procedure, without the use of columns and filters or laborious repeated pipetting and centrifugation
- No toxic phenol/chloroform required
- No ethanol in wash buffer to avoid possible residual ethanol in elution buffer
- Ultra-pure DNA ready for PCR, restriction digestion and other downstream DNA applications
- Low cost
DNA Purification Kits
RNA Purification Kits
BcMag™ Magnetic Beads
BcMag™ magnetic beads are uniform, monodisperse, spherical, core-shell superparamagnetic beads consisting of a nanoscale superparamagnetic iron oxide core completely encased in a high purity silica shell. Due to its superior mechanical and chemical stability, silica is an excellent chromatographic separation medium for purifying target molecules.
Specifications of BcMag™ Magnetic Beads
| Composition |
Silica-coated iron oxide |
| Bead Size (diameter) |
~1 µm (cv ≤ 5%); ~5 µm (cv ≤ 10%) |
| Number of Beads (beads/mg) |
~1.7 x 10⁸ (1µm); ~5 x 10⁶ (5 µm) |
| Surface Area |
>100 m²/g |
| Magnetization |
~40 - 45 EMU/g |
| Type of Magnetization |
Superparamagnetic |
| Effective Density |
2.5 g/ml |
| Stability (pH) |
4.0-10 (long-term); 3< >11 (short-term) |
| Stability (temperature) |
4°C - 140°C |
| Stability (organic solvents) |
Most organic solvents |
Features and Advantages of BcMag™ Magnetic Beads
- Fast (60% ion content)
- Very low nonspecific binding
- Organic solvent resistant
- High binding capacity
- Broad range of working temperature
- Easy sterilization and autoclavable
BcMag™ Quick Gene Mutation Finder kits
Amerigo Scientific offers BcMag™ Quick Gene Mutation Finder Kits for quick and efficient one-step isolation of DNA mutation from PCR products or amplified genomic DNA. The isolated mutated DNA can be quickly detected. This magnetic kit consists of proprietary BcMag™ Heteroduplex DNA Finder Magnetic Beads and binding/wash buffer, and does not require laborious repeat of pipetting and centrifugation.
Features and Advantages
- Quick, easy high-throughput procedure without the use of columns and filters or laborious repeated pipetting and centrifugation
- Identification of DNA mutations by highly efficient, one-step procedure
- Scalable - easy sample size adjustment and high-throughput screening of DNA mutations
- Reproducible results for reliable DNA mutation detection
Products
BcMag™ Heteroduplex DNA Finder Magnetic Beads
BcMag™ Heteroduplex DNA Finder Magnetic Beads are designed for high-efficient isolation of heteroduplex DNA. The beads work best for DNA fragments between 100bp to 600bp.
Specifications
| Composition |
Silica-coated iron oxide |
| Bead Size |
1 µm diameter |
| Number of Beads |
~1.7 x 10⁸ beads/mg |
| Surface Area |
~100 m²/g |
| Magnetization |
~40 EMU/g |
| Type of Magnetization |
Superparamagnetic |
| Effective Density |
2.5 g/ml |
| Concentration |
20 mg/ml (8 mM MgCl2, 50 mM, NaCl, 10 mM Tris, pH 8,50% glycerol) |
| Storage |
Store at –20°C upon receipt |
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