| Product Name |
LudgerSep C Buffer x4 Concentrate |
LudgerSep N Buffer x40 Concentrate |
LudgerSep R BPT solvent x10 concentrate |
| Application |
Used in WAX-HPLC analysis of labeled glycans |
For preparation of LS-N buffer (50 mM ammonium formate buffer, pH 4.4) used in amide or HILIC-HPLC analysis of labeled glycans |
For preparation of butylamine/ orthophosphoric acid/ tetrahydrofuran solvent (BPT) used in monosaccharide HPLC analysis. |
| Description |
2.0 M ammonium formate buffer/ solution pH 9.0 |
50 ml of x40 LS-N buffer (2.0 M ammonium formate buffer/solution) |
50 mL of x10 LS-BPT solvent |
| Usage |
Dilute the whole contents of the bottle (50mL) with 150 mL HPLC grade water, then add 50 mL acetonitrile to make LS-C solvent (500 mM ammonium formate H 9, 20% acetonitrile v/v).
The 50 mL of x4 buffer will make 250 mL of LS-C solvent. |
Dilute with de-gassed HPLC grade water (use 1 part of x40 buffer to 39 parts of water) to make LS-N buffer (50 mM ammonium formate, pH 4.4). The 50 ml of x40 buffer will make 2 liters of LS-N buffer. |
Dilute with de-gassed HPLC grade water use 1 bottle of LS-R-BPTX10 solvent to 450 mL of water.
The 50 mL of x10 solvent will make 500 mL of BPT solvent. |
| Storage |
Store unopened bottle below 25 °C. |
Store unopened bottle below 25 °C. |
Store unopened bottle at 4 °C. |
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