Glycosylation is one of the most important post-translational modifications of eukaryotic cell proteins, influencing the characteristics of proteins such as folding, stability, interactions, and solubility. The major classes of glycosylation include N-linked glycans, O-linked glycans, glycolipids, and glycosaminoglycans. Glycan-modified proteins are involved in a wide range of key biological and physiological processes, such as cellular communication, gene expression, cellular immunity, growth, and development.
Considering the important role of glycan-modified proteins in molecular encounters, glycan analysis is increasingly applied in biological research, clinical analysis, and pharmaceutical biotechnological production. The common analytical methods, including high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), mass spectrometry (MS), and isoelectric focusing (IEF), are used to analyze intact glycoproteins, glycopeptides, released glycans, and monosaccharides. New microarray approaches are evolving rapidly to meet the emerging needs of glycobiology.
At Amerigo Scientific, a comprehensive range of products are supplied for your glycan and glycoproteomics research. This includes kits for release and labelling of monosaccharides or sialic acids, labelling kits, clean up cartridges, glycobiology enzymes and standards, etc.
The Velocity SPE vacuum manifold system is a 96 well microplate format system which enables analysts to process up to 96 samples simultaneously. Use of a vacuum manifold for sample clean up speeds up processing times. The Velocity vacuum manifold system is compatible with cartridges or plates, and is a valuable tool for your lab.