• Amerigo Scientific Instrument
  • The traditional methods of inserting genes into vectors are based on DNA cleavage by restriction endonuclease and then ligation by DNA ligase. However, this approach to DNA engineering is time-consuming and relatively inefficient. Ligation Independent Cloning (LIC) technique is developed as an alternative to restriction endonuclease/ligase cloning. LIC provides a simple and cost-effective tool for producing many constructs of a single target or multiple targets in parallel without the need to select specific restriction enzymes for each gene.

    T4 DNA polymerase is a template-dependent DNA polymerase that has 3’-exodeoxyribonuclease activity, but lacks 5’ to 3’ exodeoxyribonuclease activity. LIC uses the 3´-exodeoxyribonuclease activity of T4 DNA polymerase to assemble DNA molecules by non-covalent complementation of single-stranded DNA of the insert and vector. Briefly, LIC relies on about 10- to 15-nucleotide complementary 3′ overhangs at the ends of a PCR-amplified DNA fragment and a linearized vector to make a stable hybridization product that can be readily used to transform host organisms without ligation. LIC based on T4 DNA polymerase cloning is strictly sequence-dependent as it requires the presence or absence of specific nucleotides at certain locations in the overlapping region.


    pColiExpress™ LIC Cloning & Expression Kits are a highly efficient, versatile and fast system of DNA cloning vectors for protein expression in E. coli. All family systems are based in directional LIC technology, a rapid procedure that provides high-cloning efficiency and the fast production of a large quantity of any desired proteins.

    Advantages & Features

    • Ready-to-use vectors.
    • Highly efficient Cloning System with Higher Protein Expression levels.
    • Special design that allows the Cell to keep larger numbers of copies than other plasmids with ori pBR322.
    • Linearized vector: ready for ligation with your PCR amplified with the recommended Primers.
    • Minimum background: lower than 1%.
    • Time-saving protocol: avoids any step required after PCR.
    • Cost avoidance: avoids the use of expensive Phosphorylated Primers.
    • Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase.
    • Risk-free: product covered by our Quality 100% Guarantee.

    Product Range

    • LIC technology based Bacterial Expression Kits
    Product Name pColiExpress™ I pColiExpress™ II pColiExpress™ III pColiExpress™ IV
    Catalog Number BE001 BE005 BE010 BE015
    5'His6 Tag      
    3'His6 Tag  
    Periplasmic expression      
    5'cmyc Tag      
    HRV protease cut site      
    Resistance Marker-Ampicillin
    T7 promoter
    Low copy number (ori pBR322)
    Strain Protein
    BL21 (DE3)
    BL21 (DE3) pLys

    • T4 DNA Ligase based Bacterial Expression Kits
    Product Name Applications
    pColiExpress™ I Cloning & Expression Kit
    • Cloning of PCR fragments for subsequent expression of proteins in E. coli.
    • Expression of Proteins under the control of the T7 promoter.
    • Protein expression in BL21 (DE3) or BL21 (DE3) (pLys).
    pColiExpress™ II Cloning & Expression Kit
    pColiExpress™ III Cloning & Expression Kit
    pColiExpress™ IV Cloning & Expression Kit

    • Competent Cells
    Product Name Applications
    BL21 (DE3) Competent Cells Protein Expression using the T7 Expression System.

    Online Inquiry

    Note: If you don't receive our verification email, do the following:

  • Copyright © Amerigo Scientific. All rights reserved.