Complementary DNA (cDNA) is DNA synthesized from an RNA template. We provide a wide range of tissue cDNAs for the research market, including cDNAs of human healthy and diseased tissue, and cDNAs of mouse, rat, monkey, and plant tissues.

By modified guanidine thiocyanate techniques, total RNAs are isolated from a variety of tissues. After RNA isolation, the first strand cDNAs are synthesized by MMLV reverse transcriptase with low RNase H activity. Our cDNAs can be directly used as a template for polymerase chain reaction (PCR) or real-time PCR, as well as for gene discovery or gene expression analysis.

Features

  • Ready to use for PCR
  • Oligo dT primer used to ensure the presence of the entire 3′ end of cDNA
  • 12 kb PCR amplicon successfully obtained with some templates indicating intact cDNAs
  • cDNAs from various tissues of a variety of species
  • Documentation of tissues’ clinical histories available

Applications

  • Immediate PCR Amplification of known genes
  • Verification of genetic mutation
  • Comparison of a specific gene between different tissues
  • Analysis of mRNA alternative splicing
  • Gene cloning and target sequencing
Products (385)
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C4434567-R
  • Size: 100 rxn
  • Catalog Number: C002096834BIO
C4434567
  • Size: 100 rxn
  • Catalog Number: C002096833BIO
C4334566-R
  • Size: 100 rxn
  • Catalog Number: C002096832BIO
C4334566
  • Size: 100 rxn
  • Catalog Number: C002096831BIO
C4534565-R
  • Size: 100 rxn
  • Catalog Number: C002096830BIO
C4234565-R
  • Size: 100 rxn
  • Catalog Number: C002096828BIO
C4234565
  • Size: 100 rxn
  • Catalog Number: C002096827BIO
C4734565-R
  • Size: 100 rxn
  • Catalog Number: C002096826BIO
C4734565
  • Size: 100 rxn
  • Catalog Number: C002096824BIO
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