Complementary DNA (cDNA) is DNA synthesized from an RNA template. We provide a wide range of tissue cDNAs for the research market, including cDNAs of human healthy and diseased tissue, and cDNAs of mouse, rat, monkey, and plant tissues.

By modified guanidine thiocyanate techniques, total RNAs are isolated from a variety of tissues. After RNA isolation, the first strand cDNAs are synthesized by MMLV reverse transcriptase with low RNase H activity. Our cDNAs can be directly used as a template for polymerase chain reaction (PCR) or real-time PCR, as well as for gene discovery or gene expression analysis.

Features

  • Ready to use for PCR
  • Oligo dT primer used to ensure the presence of the entire 3′ end of cDNA
  • 12 kb PCR amplicon successfully obtained with some templates indicating intact cDNAs
  • cDNAs from various tissues of a variety of species
  • Documentation of tissues’ clinical histories available

Applications

  • Immediate PCR Amplification of known genes
  • Verification of genetic mutation
  • Comparison of a specific gene between different tissues
  • Analysis of mRNA alternative splicing
  • Gene cloning and target sequencing
Products (385)
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C1234003-10
  • Size: 10 rxn
  • Catalog Number: C002096483BIO
C1234004
  • Size: 40 rxn
  • Catalog Number: C002096515BIO
C1234010
  • Size: 40 rxn
  • Catalog Number: C002096516BIO
C1234013-10
  • Size: 10 rxn
  • Catalog Number: C002096485BIO
C1234020-10
  • Size: 10 rxn
  • Catalog Number: C002096486BIO
C1234024-10
  • Size: 10 rxn
  • Catalog Number: C002096487BIO
C1234035
  • Size: 40 rxn
  • Catalog Number: C002096517BIO
C1234036-10
  • Size: 10 rxn
  • Catalog Number: C002096489BIO
C1234039
  • Size: 40 rxn
  • Catalog Number: C002096518BIO
C1234040-10
  • Size: 10 rxn
  • Catalog Number: C002096488BIO

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