• Amerigo Scientific Instrument
  • UniSil® hydrophilic interaction liquid chromatography (HILIC) silica gel is a new generation of HILIC media developed through unique surface passivation and bonding technologies with multiple functions such as hydrophilic, separation and distribution. Moreover, solutions containing up to 60% H2O can be used as the mobile phase of the medium.

    Many high polarity substances have too weak retentivity in reversed-phase chromatography to be separated (k≈0), but can be easily separated using hydrophilic interaction chromatography. Compared to reversed silica gel, UniSil® HILIC silica gel is a better choice for highly polar and hydrophilic molecules, especially strongly alkaline chemicals, with a high proportion of volatile mobile phases that can improve the sensitivity of LC-MS.

    Products

    Product Name Cat. No. Particle Diameter Pore Size Size
    UniSil® 5-120 HILIC-T 19012-050012 5 μm 120 Å 10g; 50g; 100g; 500g; 1kg
    UniSil® 5-120 HILIC-2M 19011-050012 5 μm 120 Å 10g; 50g; 100g; 500g; 1kg
    UniSil® 5-120 Amide 19017-050012 5 μm 120 Å 10g; 50g; 100g; 500g; 1kg
    UniSil® 10-120 HILIC-T 19012-100012 10 μm 120 Å 100g; 500g; 1kg; 5kg; 10kg; 20kg
    UniSil® 10-120 HILIC-2M 19011-100012 10 μm 120 Å 100g; 500g; 1kg; 5kg; 10kg; 20kg
    UniSil® 10-120 Amide 19017-100012 10 μm 120 Å 100g; 500g; 1kg; 5kg; 10kg; 20kg

    Applications

    UniSil® HILIC silica gels are especially suitable for separation of highly polar molecules that are typical challenges for convention reversed phase chromatography.

    • HILIC-T - separation of oligosaccharides, glycine and chemicals with similar molecule
    • HILIC-2M - separation of melamine, streptomycin and chemicals with similar molecule
    • Amide - separation of stevioside and chemicals with similar molecules

    Application Examples

    Application of UniSil® 5-120 HILIC-2M for using pure H2O as mobile phaseApplication of UniSil® 5-120 HILIC-2M for using pure H2O as mobile phase


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