UniSil® hydrophilic interaction liquid chromatography (HILIC) silica gel is a new generation of HILIC media developed through unique surface passivation and bonding technologies with multiple functions such as hydrophilic, separation and distribution. Moreover, solutions containing up to 60% H2O can be used as the mobile phase of the medium.
Many high polarity substances have too weak retentivity in reversed-phase chromatography to be separated (k≈0), but can be easily separated using hydrophilic interaction chromatography. Compared to reversed silica gel, UniSil® HILIC silica gel is a better choice for highly polar and hydrophilic molecules, especially strongly alkaline chemicals, with a high proportion of volatile mobile phases that can improve the sensitivity of LC-MS.
Products
Applications
UniSil® HILIC silica gels are especially suitable for separation of highly polar molecules that are typical challenges for convention reversed phase chromatography.
- HILIC-T - separation of oligosaccharides, glycine and chemicals with similar molecule
- HILIC-2M - separation of melamine, streptomycin and chemicals with similar molecule
- Amide - separation of stevioside and chemicals with similar molecules
Application Examples
Application of UniSil® 5-120 HILIC-2M for using pure H2O as mobile phase
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