dna ladders

Nucleic Acid Standards

Nucleic acid fragment size is usually reported in "nucleotides", "base pairs (bp)" or "kb” depending upon whether single- or double-stranded nucleic acid has been separated. Fragment size is usually determined by comparison with nucleic acid standards that contain fragments of known length.

Nucleic acid standards, also known as markers or ladders, are a set of standards that are used to identify the approximate size of a molecule run on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to its mobility through a gel matrix.

Factors considered in selecting an appropriate ladder for a given sample include the type of ladder, fragment structure and conformation, the number of fragments and their separation patterns, and the suitability of the ladder and dyes to the gel respectively.

DNA Ladders

Amerigo Scientifc offers ideal DNA ladders for quantitative estimation of DNA mass in gels. OneMARK series is a ready-to-use format containing the fluorescent DNA stain and tracking dyes for direct loading onto unstained gels.

Product Name Range Size
BH 50bp DNA Ladder RTU 50-1,500 bps 500 μl
BH 100bp DNA Ladder RTU 100-1,500 bps 500 μl
BH 100bp DNA Ladder H3 RTU 100-3,000 bps 500 μl
BH 1Kb plus DNA Ladder RTU 100-10,000 bps 500 μl
BH OneMARK 100 RTU 100-3,000 bps 600 μl
BH OneMARK B Plus DNA Ladder RTU 100-10,000 bps 600 μl

Benefits

  • Versatile: size range from 50 bp to 10 Kb
  • Consistency: sharp and clear bands for reliable results
  • Convenient: optimized size ranges for analysis

DNA Ladders Benefits

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