Chromatography systems are versatile and indispensable tools for separation, analysis, and purification in a wide range of scientific disciplines. These systems are capable of separating complex mixtures and providing detailed information about the components within them, allowing researchers to gain insight into the components in a sample and their relative concentrations. A chromatography system consists of a stationary phase and a mobile phase. The stationary phase can be a solid or liquid material and is usually packed in a chromatography column or coated on a solid support. The mobile phase, usually a liquid or gas, carries the sample through the stationary phase. When a mixed sample interacts with the stationary phase, the different components of the sample are separated based on factors such as different size, charge, polarity, or affinity.

A variety of chromatography technologies are developed with its own unique principles and applications. Liquid chromatography refers to the separation of components in a liquid mobile phase used to analyze complex mixtures, identify compounds, and quantify their concentrations. Liquid chromatography can be further classified into high-performance liquid chromatography (HPLC), ion-exchange chromatography, and size-exclusion chromatography according to the stationary phase characteristics and separation principle. Gas chromatography separates volatile compounds that pass through a gaseous mobile phase utilizing vapor pressure, boiling point, and affinity for the stationary phase. This technique is extensively used for the characterization and analysis of volatile organic compounds (VOCs), environmental pollutants, and other volatile mixtures. Thin Layer Chromatography (TLC) involves applying the sample to a thin layer of stationary phase and then achieving separation by the rising action of a liquid mobile phase, which is commonly used for the characterization and identification of compounds. Affinity chromatography utilizes specific binding interactions between a target molecule and a ligand immobilized on a stationary phase to purify biomolecules such as proteins and enzymes.

Chromatography is an essential separation method for life science research and the biopharmaceutical industry, with applications ranging from bench-scale purification to process development. In the field of fundamental research, chromatography is commonly employed in proteomics, metabolomics, and drug development, such as drug discovery, quality control, and pharmacokinetics studies. Moreover, chromatography plays a crucial role in environmental monitoring, food and beverage analysis, as well as forensic testing, such as drug testing, toxicology analysis, and trace evidence analysis.

Amerigo Scientific offers flexible, scalable, and easy-to-use chromatography instruments. A wide range of chromatography systems can be chosen to meet special research needs. With our high-quality chromatography components and accessories, we will continue to support your efforts to improve productivity and process efficiency.