UniMab® Protein A Affinity resins are designed to raise the purification efficiency of monoclonal antibodies (mAb) and recombinant Fc fragment proteins. The resins are based on rigid, mono-sized polymethacrylate matrices with good surface hydrophilicity, minimal non-specific binding and high mechanical strength for fast flow operations. With excellent FC binding selectivity and base stability, UniMab® Protein A Affinity resins have proven its strong properties in many successful applications including bioprocess purification of mAb-drugs in phase III.
Products
| Product Name |
Cat. No. |
Particle Diameter |
Pore Size |
Size |
| UniMab 50 |
17010-050100 |
50 μm |
|
30ml; 100ml; 500ml; 1L; 10L |
Highlights
- Suitable for columns with higher bed height
- High dynamic binding capacity (DBC) and high recovery yield
- Minimal non-specific binding
- Superior alkaline stability
- High reproducibility
- Low concentration host cell protein (HCP) and low level of protein A leachate
Features
| Matrix |
Mono-sized Polymethacrylate |
| Ligand |
Engineered rProtein A |
| Particle size, µm |
50 |
| Loading, mg human IgG/ml packing (4 min residence time) |
>35 |
| Maximum pressure, psi (bar, MPa) |
116 (8,0.8) |
| Maximum flow rate, cm/h |
800 |
| Protein A leachate |
< 5 ng/ml |
| pH stable range |
3-12 |
| CIP cleaning |
0.1-0.5M NaOH |
| Temperature, °C |
4-40 |
| Storage |
20% Ethanol, 2-8 °C |
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