Gluten-related Disorder Products
Gluten-related disorders (GRD) are a group of immune-mediated diseases with diverse manifestations triggered by the ingestion of gluten. Celiac disease is a prevalent GRD where ingestion of gluten drives a chronic inflammation in the small intestine of genetically predisposed individuals. Transglutaminase 2 (TG2) catalyzes highly specific deamidation of glutamine side chains in gluten peptides. This modification improves the binding of the peptides to HLA-DQ2 and HLA-DQ8 molecules associated with celiac disease, which is considered essential for the T cell mediated immune response against gluten in this disease. TG2 is also the primary autoantigen of celiac disease, and the autoantibody production is dependent on the gluten intake. Assessment of serum anti-TG2 antibodies has become an important tool in diagnosis.
In addition to intestinal diseases, gluten may cause extra-intestinal disease manifestations such as the skin disorder dermatitis herpetiformis and various neurological conditions, the most prevalent being gluten ataxia and gluten neuropathy. These conditions can present with or without small intestinal symptoms and anti-TG2 autoantibodies. Dermatitis herpetiformis patients have antibody populations primarily recognizing transglutaminase 3 (TG3, also known as epidermal transglutaminase) while gluten ataxia patients produce antibodies against transglutaminase 6 (TG6, also known as neuronal transglutaminase), which shared enzymatic properties with both TG2 and TG3. TG3 and TG6 are now considered to be the main autoantigens in dermatitis herpetiformis and gluten ataxia, respectively, and these corresponding antibody populations are useful for the diagnosis of diseases.
Gluten-related Disorder Antigens
Neuronal Transglutaminase (TG6) ELISA Kits
Amerigo Scientific offers ZediXclusive anti-TG6 IgA or IgG ELISA kits with benefits:
- No need for background subtraction. Up to 40 duplicate serums are measured using one kit.
- The calibrators are adapted, so that the more relevant lower titers are covered.
- Redefined cutoffs are based on serum from 38 blood donors (equally distributed by sex and age) and 86 patients with various neurological disorders.
Non-celiac Disease Antigens
Wheat dependent exercise induced anaphylaxis (WDEIA) is a rare and life-threatening IgE-mediated allergy to omega-5 gliadin, a protein component of gluten. In addition to omega-5-gliadin, other wheat allergens may be implicated in WDEIA. In addition, co-factors such as exercise, alcohol, menstruation, and aspirin/NSAIDs may reduce the threshold of anaphylaxis.
Amerigo Scientific offers highly pure omega-5 gliadin, which are produced recombinantly and purified through multi-step chromatography and precipitation.
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