Gluten-related disorders (GRD) are a group of immune-mediated diseases with diverse manifestations triggered by the ingestion of gluten. Celiac disease is a prevalent GRD where ingestion of gluten drives a chronic inflammation in the small intestine of genetically predisposed individuals. Transglutaminase 2 (TG2) catalyzes highly specific deamidation of glutamine side chains in gluten peptides. This modification improves the binding of the peptides to HLA-DQ2 and HLA-DQ8 molecules associated with celiac disease, which is considered essential for the T cell mediated immune response against gluten in this disease. TG2 is also the primary autoantigen of celiac disease, and the autoantibody production is dependent on the gluten intake. Assessment of serum anti-TG2 antibodies has become an important tool in diagnosis.
In addition to intestinal diseases, gluten may cause extra-intestinal disease manifestations such as the skin disorder dermatitis herpetiformis and various neurological conditions, the most prevalent being gluten ataxia and gluten neuropathy. These conditions can present with or without small intestinal symptoms and anti-TG2 autoantibodies. Dermatitis herpetiformis patients have antibody populations primarily recognizing transglutaminase 3 (TG3, also known as epidermal transglutaminase) while gluten ataxia patients produce antibodies against transglutaminase 6 (TG6, also known as neuronal transglutaminase), which shared enzymatic properties with both TG2 and TG3. TG3 and TG6 are now considered to be the main autoantigens in dermatitis herpetiformis and gluten ataxia, respectively, and these corresponding antibody populations are useful for the diagnosis of diseases.
|Recombinant TG2 (E. coli)||250 µg; 1 mg|
|Recombinant TG2 (insect cells)||250 µg; 1 mg|
|Human epidermal transglutaminase (TG3, recombinantly produced in insect cells)||200 µg|
|Human neuronal transglutaminase (TG6, recombinantly produced in insect cells)||250 µg; 1 mg|
|Deamidated alpha2-gliadin||5 mg|
|Alpha2-gliadin, 33mer (aa 56-88)||5 mg|
|Deamidated alpha2-gliadin, 33mer||5 mg|
|Alpha2-gliadin, 33mer (aa56-88), TAMRA labeled||5 mg|
|Alpha2-gliadin, 33mer (aa56-88), TAMRA and Biotin labeled||5 mg|
Amerigo Scientific offers ZediXclusive anti-TG6 IgA or IgG ELISA kits with benefits:
|ZediXclusive TG6-ab ELISA (IgA)||1 Kit/96 wells|
|ZediXclusive TG6-ab ELISA (IgG)||1 Kit/96 wells|
Wheat dependent exercise induced anaphylaxis (WDEIA) is a rare and life-threatening IgE-mediated allergy to omega-5 gliadin, a protein component of gluten. In addition to omega-5-gliadin, other wheat allergens may be implicated in WDEIA. In addition, co-factors such as exercise, alcohol, menstruation, and aspirin/NSAIDs may reduce the threshold of anaphylaxis.
Amerigo Scientific offers highly pure omega-5 gliadin, which are produced recombinantly and purified through multi-step chromatography and precipitation.
|Omega-5 gliadin||1 Kit/96 wells|