For the biotechnological production of recombinant proteins, different prokaryotic and eukaryotic expression systems are used. Among those, bacterial expression systems are especially attractive because of their ability to grow rapidly and at high density on inexpensive substrates, their well-characterized genetics, and the availability of an increasingly large number of cloning vectors and mutant host strains.
In general, the determination of the expression system depends on the target protein to be expressed. Many bacterial systems cannot modify proteins after translation, such as glycosylation. If the post-translational modification is essential for bioactivity, bacterial expression systems should not be used to produce the proteins. Alternative hosts such as yeast, filamentous fungi, or insect and mammalian cells can be used for this application.
Escherichia coli (E. coli) expression system dominates the bacterial expression systems and is the first choice for laboratory investigations and initial development in commercial activities, because of its extensive research, easy culture and operation, fast growth rate and high yield. Other bacterial systems such as Streptomyces, coryneform bacteria, and Lactococcus lactis offer more choices of bacterial expression systems. But these bacteria expression systems are not often used due to unknown information about regulation and mechanisms, as well as the fact that there are no commercial vectors and promoter systems.
Amerigo Scientific offers bacterial systems that allow efficiently cloning DNA fragments and the quick production of a large quantity of any desired protein.
Note: If you don't receive our verification email, do the following: