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Amerigo Scientific offers a new generation of boronic acid affinity packings in which boronic acid forms a coordination compound with cis-diol for separation and purification. Under alkaline conditions, boronic acid functional group interacts with the cis isomer of a 1,2-diol forming a stable five-membered cyclic adduct on the stationary phase. Under acidic conditions, the adduct ring opens releasing the target molecules.

Our boronic acid affinity media are employed in the separation and purification of compounds containing cis-diol groups such as glycoproteins, nucleosides, nucleotides, sugars, etc.

Boronic Acid Affinity Chromatography Resins

Products

Product Name Cat. No. Particle Diameter Pore Size Size
UniPB-80L 04000-080001 80 μm 30ml; 100ml; 1L

Characteristics

Separation principle Boronic acid affinity capture
Substrate Poly(methyl methacrylate), or PMMA
Ligand Phenyl boronic acid
Particle size, µm 80
Pore size, Å 1000
Purification stage Capture, moderate purification
Characteristics High flow rate, low back pressure, stable in cleaning
Maximum pressure, MPa 0.5
Recommended linear flow rate, cm/ h 150-750
pH stable range 2-12
CIP cleaning 0.1-0.5 M NaOH
Storage 20% ethanol, 2-8 °C

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