• Amerigo Scientific Instrument
  • Amerigo Scientific offers a new generation of boronic acid affinity packings in which boronic acid forms a coordination compound with cis-diol for separation and purification. Under alkaline conditions, boronic acid functional group interacts with the cis isomer of a 1,2-diol forming a stable five-membered cyclic adduct on the stationary phase. Under acidic conditions, the adduct ring opens releasing the target molecules.

    Our boronic acid affinity media are employed in the separation and purification of compounds containing cis-diol groups such as glycoproteins, nucleosides, nucleotides, sugars, etc.

    Boronic Acid Affinity Chromatography Resins


    Product Name Cat. No. Particle Diameter Pore Size Size
    UniPB-80L 04000-080001 80 μm 30ml; 100ml; 1L


    Separation principle Boronic acid affinity capture
    Substrate Poly(methyl methacrylate), or PMMA
    Ligand Phenyl boronic acid
    Particle size, µm 80
    Pore size, Å 1000
    Purification stage Capture, moderate purification
    Characteristics High flow rate, low back pressure, stable in cleaning
    Maximum pressure, MPa 0.5
    Recommended linear flow rate, cm/ h 150-750
    pH stable range 2-12
    CIP cleaning 0.1-0.5 M NaOH
    Storage 20% ethanol, 2-8 °C

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