Sphericalplate 5D

The 3D Cell Culture Revolution

Born from the clinical need of cell transplantation and stem cell biology, Amerigo Scientific offers the Sphericalplate 5D (SP5D). Developed with patented technology, this 24-multiwell plate allows the generation of highly standardized cell spheroids, freely scalable from hundreds for the laboratory to millions for clinical use. Aiming to achieve the highest medical and regulatory standards, seamless translation into diagnostic or clinical use is enabled.

The 5D of Sphericalplate 5D, the fifth dimension, can be defined as the communication between cells: the critically determined shape and size of microwells create an environment as physiological as possible. In addition, a special nano-coating prevents cells from attaching to the surface. This results in the formation of uniform spheroids with an undisturbed communication of the cells with each other, preventing wrong signals that would induce unwanted gene expression/differentiation.

OUR TECHNOLOGY

  • Uniformly sized spheroids
  • Physiological, standardized and easy spheroid formation by optimized niche geometry and surface
  • Convenient upscaling without loss of spheroid quality
  • Ability to translate into diagnostic or clinical use
The 3D Cell Culture Revolution
  • Safety First
  • Effortless upscaling of your cell spheroids without losing control over cell differentiation and spheroid size
  • Medium change in your culture is rather simple and can be automated as well
  • The safety profile of the SP5D reduces the risk of unintended tumorigenesis to a minimum which is a prerequisite for all translational approaches
Safety First
  • Size and Space Matters

Due to our patented geometry:

  • Precise control of spheroid size resulting in higher standardization & reproducibility
  • High quantity of uniformly sized spheroids (9000/plate)
  • Identical diffusion conditions for all spheroids
  • Unique physiological spheroid formation with rounded bottoms
Size and Space Matters
  • Benefits at a Glance

Benefits at a Glance

APPLICATIONS

The SP5D allows the generation of uniform, size controlled cell clusters required for a wide variety of research and diagnostic applications, supporting the transition from bench to bedside, including scalable, reliable and safe cell therapy applications. The SP5D features round-bottomed microwells and is covered with a high-end ultra-low attachment coating to achieve perfect cell aggregation. 3D cell culture protocols using the SP5D are highly scalable, as a single plate can hold up 9000 spheres. Scalable solutions on the market do not meet the quality criteria required for safe clinical use, so our SP5D fills this gap.

APPLICATIONS

  • Cells Successfully Cultivated in SP5D
human embryonic stem cells human breast cancer cell line (MCF-7)
mouse embryonic stem cell line (HM-1) human A549 cell line from lung carcinoma
human bone marrow-derived mesenchymal stromal cells mouse 3T3 fibroblast cell line
human adipose-derived mesenchymal stromal cells human umbilical vein endothelial cell line (huVEC)
human islet cells human liver carcinoma cell line (HepG2)
rat islet cells human pluripotent stem cell line (Hs181)
mouse islet cells human osteosarcoma cell line (Saos-2)
human glioblastoma cell line human caucasian fetal lung cell line (WI 38)
human prostate cancer cell line human amniotic epithelial cells (hAEC)
human adrenal gland cancer cell line induced pluripotent stem cells (iPSCs)
human breast cancer line (BT20)  

Documents

SP5D Manual
SP5D & THUNDER Technologies
Application Note: SYNENTEC
Application Note: CELLDYNAMICS
Application Note: CELLINK


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