BcMag™ Carboxy-terminated Magnetic Beads Conjugation Buffer Kit

BcMag™ Carboxy-Terminated Magnetic Beads are uniform, silica-based superparamagnetic beads coated with high density carboxyl functional groups on the surface. The beads are used to covalently conjugate primary Amine- containing ligands via a stable amide bond. BcMag™ Carboxy-Terminated Magnetic Beads are most suitable for conjugation of large proteins. BcMag™ Long-arm Carboxy-terminated Magnetic Beads are recommended for conjugation of small peptides because the long-arm (21-atom) hydrophilic linker may reduce steric hindrance.

• Recommended coupling conditions: pH 4.5–6, 25°C, 2–16 h.
• Stable covalent bond with minimal ligand leakage
• Produces reusable immunoaffinity matrices
• Low nonspecific binding
• Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads
• Applications: Purification for Antibodies, Proteins/Peptides, DNA/RNA Cell sorting, Immunoprecipitation

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