Chiral compounds are widespread in nature and play key roles in biochemical processes and the development of life. Most biological molecules such as amino acids, sugars, and nucleic acids are chiral. Many synthetic compounds such as some drugs are also chiral. A chiral molecule exists in two stereoisomers that are mirror images of each other, called enantiomers. Chiral chromatography is a common technique for the separation of chiral compounds or enantiomers, which is widely used in pharmaceutical, fine chemical, food science, environmental analysis and other fields. The role of chirality in drug design is important, because of the chirality determines biological interactions, safety, and efficacy of drugs. Therefore, the confirmation and control of enantiomeric purity, as well as the determination of individual enantiomeric drug molecules, are very necessary during drug discovery and development.
Chiral chromatography is usually performed in classical elution mode. The mobile phase is continuously passed through a column containing the stationary phase. The mobile phase can be gaseous, liquid or a supercritical fluid. The key to chiral chromatography is enantiomeric selectivity, which is usually provided by a chiral stationary phase. Alternatively, enantiomeric selectivity can be achieved by a chiral mobile phase or by the addition of a chiral selector to the eluent. Preferential interaction of one of the enantiomers in a mixture with the stationary phase results in a reduced migration velocity relative to the other enantiomer and a separated elution from the column. Among many methods used to separate and analyze enantiomerically pure compounds, high performance liquid chromatography (HPLC) based on chiral stationary phase is the most commonly used, accurate, and effective. Common chiral stationary phases include Pirkle-type, ligand-exchange-type, molecularly-imprinted, as well as chiral stationary phases based on polysaccharides, macrocyclic antibiotics, proteins, cyclodextrins, crown ethers, cyclofructins, synthetic polymers, etc.
Product Range
Amerigo Scientific offers a wide range of high-quality chiral chromatography columns in a variety of specifications for analytical and/or small-scale separation, as well as commercial separation of enantiomers. Our columns with durability and long service life offer excellent resolution of racemates, and rapid and easy method development, which are essential tools in the pharmaceutical industry.
Our chiral HPLC columns include traditional polysaccharide-based chiral columns, solvent-resistant immobilized type chiral columns, and chiral columns based on protein stationary phases. Our specialty chiral columns include HPLC columns of various specifications based on crown ether stationary phases, anion exchange stationary phases, or ligand exchange stationary phases. In addition, we also offer chiral supercritical fluid chromatography (SFC) columns using supercritical fluid of carbon dioxide as the mobile phase. Our SPC columns provide high-resolution separation of complex mixtures, low back pressure, and low solvent consumption.
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