Immunohistochemistry (IHC) is a widely used technique that bridges immunology, histology. The basic principle of IHC is visual localization of target molecules within tissue sections by means of specific antibodies, under an appropriate signal-to-noise ratio condition. The key advantage of IHC is that it could not only analyze the anatomy of the tissue but also visualize of the spatial distribution and expression of specific antigens or cellular components in a variety of tissue sections. In particular, IHC allows colocalization of an antigen with a lesion, thereby dramatically enhancing diagnostic interpretation and understanding of the pathogenesis.
IHC can be summarized in three major steps: (1) tissue fixation, trimming, embedding, and sectioning; (2) incubation of tissue sections and the primary antibody, and then the addition of a secondary enzyme-conjugated detection antibody; (3) visualization analysis with a microscope.
IHC fills the gap between classic histopathology and molecular pathology with more stringent requirements for sensitivity and specificity. In the second step of the IHC trial, the role of the primary antibody is particularly critical. Selection of the primary antibody requires confirmation that the primary antibody is specific, selective, and reproducible for its intended use.
IHC Guaranteed Antibodies are the primary antibody products that have been successfully stained on formalin-fixed and paraffin embedded (FFPE) tissue sections. Each product is manufactured to a high standard and fully validated in immunohistochemistry with a 100% guarantee. Both monoclonal and polyclonal antibodies derived from different hosts have been professionally engineered to provide consistent specificity and sensitivity.