Oligo Synthesis Services

Oligonucleotides (oligos) are short-chain nucleotides, ranging from a few to dozens of bases in length, and serve as versatile tools in molecular biology. High-quality oligonucleotides play a critical role at every key stage of life science research. Whether for precise regulation of gene expression in basic research, targeted design in gene therapy for clinical applications, or efficient RNA screening in drug development, accurate and reliable oligonucleotides are the cornerstone of experimental success.

Amerigo Scientific provides a comprehensive suite of customized oligonucleotide synthesis services to meet diverse needs across cutting-edge fields, including gene synthesis, PCR, sequencing, development of molecular diagnostics, drug discovery, and CRISPR gene editing. Our oligonucleotide synthesis services leverage advanced synthesis technologies, premium-grade reagents, state-of-the-art automated equipment, and a stringent ISO13485 quality control system. Advanced solid-phase synthesis technology is utilized combined with rigorous quality control protocols such as 100% mass spectrometry (MS) analysis and additional detection options to ensure the high accuracy and reliability of each oligonucleotide product.

Beyond standard-length and sequence synthesis, we specialize in overcoming challenges associated with complex sequences. Our services offer 200+ chemical modifications and multiple purification options to guarantee the high-quality results you require. Backed by a technical team with extensive synthesis expertise, we provide professional guidance on sequence design and tailored modification strategies to address your specific research or application goals.

Advantages

Relying on ISO 13485-certified workshops equipped with Class 100,000 clean room and adhering to the highest industry standards, Amerigo Scientific provides precise and custom oligo synthesis services with high quality and reliability.

Advanced Equipment and High Capacity

• With advanced equipment such as proprietary synthesizers, ammonolysis instruments, microplate readers, high-performance liquid chromatography (HPLC) systems, mass spectrometers, and capillary electrophoresis systems, synthesis levels from μg to kg scale can be achieved.

High-quality Raw Material and Consumables

• Critical synthesis raw materials (e.g., CPG, monomers, dyes for modifications) are sourced from high-quality products. A stringent procurement management system ensures the stability and premium quality of raw materials.

Refined Methodology and Process

• A series of contamination prevention measures have been established for diverse detection applications, with a cross-contamination rate of less than 0.01%.
• Refined processes and comprehensive operating guidelines ensure precise control over lot-to-lot variations.
• Certificate of Analysis (COA) is provided upon request.

Rigorous Process Control

• Critical steps in synthesis are controlled through ISO-standardized management, adhering to controlled documentation throughout the process.
• Customized Standard Operating Procedures (SOPs) according to customer’s requirements.
• All critical raw materials, intermediates, and final products are monitored, enabling full traceability.
• 100% in-process monitoring via CE, MS, HPLC, and OD detection ensures consistent oligo quality, accurate quantification, and stable signal performance.

Service Types

Amerigo Scientific provides various services for the synthesis of custom DNA oligos, RNA oligos, siRNA, sgRNA, and custom next-generation sequencing oligos to meet specific research needs with precision and reliability.

FAQs