Advanced microfluidic cell culture systems have been increasingly used in tissue engineering, diagnostics, drug screening, immunology, cancer research, stem cell proliferation and differentiation, and other fields. Microfluidic technology enables dynamic cell culture in microperfusion systems.
Cells are cultured on microfluidic devices with channels that facilitate diffusion of substrates, nutrients, and reagents delivered by continuous perfusion systems. Unlike conventional cell culture methods, cell culture systems based on microfluidic technology provide precise, controllable, cost-effective, compact, integrated, and high-throughput microsystems. Microfluidic devices are capable of creating gradient concentrations of biochemical signals such as growth factors, chemokines and hormones, and replicating complex three-dimensional structures of tissues and organs.
Amerigo Scientific offers four microfluidic chips made of medical-grade plastics cycloolefin polymer (COP) and cycloolefin copolymer (COC) to meet a variety of research needs in 2D/3D cell culture.
|BE-GRADIENT||a chip for 3D cell culture under an electrochemical gradient|
|BE-FLOW||an ideal device to study in the effect of flow and mechanical stress on cell culture|
|BE-DOUBLEFLOW||an ideal device used for endothelium/epithelium barrier coculture environment where hypoxia or flux plays a role|
|BE-TRANSFLOW||an ideal chip for air liquid interface (ALI) cell culture and coculture research (skin, cornea, gut, lung)|
Our microfluidic chips are compatible with any type of optical microscopy (confocal, fluorescence, etc.) and their slide format is designed for easy handling under a microscope. The microfluidic chambers, wells and channels in our chips match 96 well plate positions, so our chips are compatible with automated microscopy.
Our microfluidic chips are compatible with all microfluidic flow control systems, such as syringe, peristaltic pumps, pressure control systems, and rocker systems.
Our microfluidic chips are made of lipophobic and thermoplastic materials that do not cause unspecific drug absorption issues. Therefore, unlike polydimethylsiloxane (PDMS) based devices, our chips can be used for fluorescent immunohistochemical detection.
Cell cultured in our chip can be easily recovered and can be used for downstream applications. (Click here to learn more)
Our microfluidic chips are made of medical grade plastic cycloolefin polymers (COP) and cycloolefin copolymers (COC).
These materials have a very low permeability to oxygen and water vapor, allowing precise control of the concentration of these gases within the microchannel and even hypoxia experiments inside our chips.
COP and COC are lipophobic materials without unspecific absorption issue, so our chips can be used for drug development and diffusion experiments.
These properties of COP and COC, including transparency in the visible and near UV range, low birefringence, high Abbe number, make them ideal materials for microscope applications.
COP and COC have remarkable chemical resistance to acids and polar solvents. The glass transition temperature of both materials is high, approaching 190 °C in some formulations.
Yes, all the chips are sterilized and stored in individual packages. We guarantee the sterility and hydrophilicity of the chips up to 6 months after shipment.
No, our chips are made of COP, a plastic material that doesn’t produce autofluorescence. But our products can be used for fluorescence experiments. Be-Transflow with a black upper piece minimizes spurious reflections of the laser in the culture well to optimize fluorescence images.
We offer two connector kits compatible with any type of microfluidic flow control system. The choice of the connector kit depends on the type of microfluidic flow control at your disposal:
• Peristaltic/syringe pump connection kit: 10 connectors and 2 m of tygon tube 3/32″ OD 1/32 ID
• Microfluidic flow control system connector kit: 10 connectors, 10 ferrules for microfluidic control system y 1,5 m FEP Tubing 1/16″ OD x 1/32″ ID.
If the shear stress is going to be applied using a rocker, there is no need for connectors, but it is necessary to use the chip lids during the experiment to avoid contamination.
Yes, COP is highly resistant to chemicals such as alcohols, acids, or aliphatic compounds.
Oxygen can be supplied to the cells via culture media. In fact, impermeability is one of the greatest advantages of our products as an organ-on-a-chip (OOC), which allows precise control of the oxygen levels in cell culture and even mimics the hypoxic environment in the body.
Yes, our chips can be customized. We will modify product properties such as the pore size of the membrane, channel size, and shape to suit your needs.
Yes. Among our customizable products, we offer chips that can be used with your own membranes. These chips without a base can be bonded in site to any base using a strong biocompatible adhesive.
Yes, our connectors can be used to connect several chips in series. This is often used to study the crosstalk between different tissues.
In addition to microfluidic chips, Amerigo Scientific offers a wide range of accessories required for microfluidic environments, including connectors to connect microfluidic flow control systems to chips, tubing with low oxygen permeability, chip lids and inlet/outlet plugs.
Accurate and stable flow control in microfluidic channels is a fundamental requirement in lab-on-a-chip devices to investigate biological systems. Amerigo Scientific offers pressure-based flow control solutions that ensure higher stability and faster responses and can even monitor and control the flow directly with the help of an additional flow sensor.