Proteomics is the large-scale or systematic study of proteins and their structure, topology, function, and interrelationships. In most biological fluids or extracts, proteins are abundant, and differences in their individual concentrations may span several orders of magnitude. Therefore, a challenge for proteomic analysis is that samples are often dominated by relatively small numbers of highly abundant proteins, whose presence masks less abundant proteins and limits the capacity and resolution of the employed separation techniques. Protein enrichment is a key step in proteomics that aims to increase the relative abundance of a specific protein or proteome in a complex biological sample such as blood, cerebrospinal fluid, saliva, cell extracts, and plant extracts. Through enrichment of proteins of interest, in-depth studies of proteomes can be achieved, particularly focusing on low-abundance proteins, post-translational modifications, and protein-protein interactions.
The principle of commonly used protein enrichment methods is based on the different properties of proteins, such as size, charge, and affinity, which allows for the selective isolation and concentration of targeted low-abundance proteins. Ideal tools or methods for enriching specific proteins should be characterized by high specificity and accuracy, low cross-reactivity to non-target proteins, high throughput, simple and easy operation, and automation. In addition, the appropriate enrichment strategy should be selected based on factors such as compatibility and repeatability of the experimental target with the downstream analysis.
Amerigo Scientific offers sensitive and reliable kits and related reagents for the enrichment of low-abundant proteins, facilitating in-depth protein analysis, disease research, and treatment development.
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