BcMag™ Thiol-Activated Magnetic Beads

BcMag™ Thiol-activated Magnetic Beads are uniform, silica-based superparamagnetic beads coated with high density Thiol functional groups (2-pyridyl disulphide) on the surface The beads are used to reversible couple thiol-containing ligands. BcMag™ thiol-activated Magnetic Beads are most suitable for conjugation of large proteins. BcMag™ Long-arm thiol- activated Magnetic Beads are recommended for conjugation of small peptides because the long-arm (17-atom) hydrophilic linker may reduce steric hindrance.

• Pre-activated and ready-to-use
• Recommended coupling conditions: pH 4–8, 4 to 25°C, 3–16 h.
• Specific isolation of cysteine proteins/peptides
• Stable covalent bond with minimal ligand leakage
• Produces reusable immunoaffinity matrices
• Low nonspecific binding
• Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads
• Applications: Cell sorting, Immunoprecipitation; Purification for Antibodies, Proteins/Peptides, DNA/RNA

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