Airlift bioreactors are one type of pneumatically agitated bioreactors and contain a draft tube (internal loop) or an external loop. In the airlift bioreactors, bubble coalescence is prevented by directing them in one direction, and shear stresses are distributed more evenly throughout the reactor, thus providing a more favorable environment for cell growth. In addition, the cyclical movement of fluid is enhanced to increase mass and heat transfer rates. Compared with traditional systems, one of the main advantages of airlift bioreactors is that the cost associated with agitation and aeration can be substantially reduced. Other advantages include ease of scale-up, simplicity of construction, low risk of contamination, efficient gas-liquid dispersion, and low power consumption. Due to these advantages, airlift bioreactors have been widely used in aerobic fermentation, biological wastewater treatment, production of single cell proteins, and cell culture.

Innovative Airlift Technology

Innovative Airlift Technology

The patented airlift technology used by CellMaker bioreactors eliminates the need for mechanical mixing and instead uses bubbles to move cells and nutrients, providing optimal aeration for rapid cell growth. This mothed of gentle agitation is suitable for microbial fermentation and cell culture. Dissolved oxygen and pH regulation, as well as accurate temperature control can be achieved optimally. The integrated heater/cooler allows temperatures from 16°C-40°C without the need for external equipment.

CellMaker single-use airlift technology uses disposable bioreactor bags to reduce the risk of culture contamination and the downtime between operations. CellMaker bioreactors can work in volumes ranging from 1.5L to 50L, offering a relatively low footprint with stand-alone, compact systems.

CellMaker Airlift Bioreactors

Amerigo Scientific offers Regular, Plus and Low Flow CellMaker Controllers available with working volumes from 1.5L to 50L. CellMaker bioreactor systems are designed to improve and simplify your cell culture and fermentation processes with batch-to-batch reproducibility and volume efficiency.

Product Description
CellMaker Regular Controller CellMaker Regular Controller enables excellent temperature and airflow control via an easy-to-use touchscreen interface.
CellMaker Low Flow Controller CellMaker Low Flow Controller delivers very low air flow (from 0.06 to 0.25 VVM) for gentle airlift mixing of cells, leading to the rapid and efficient generation of cells.
Dual Regular Controller CellMaker Regular Controller is available as a dual unit with the option to run identical or different processes at the same time in either two x 8L units, two x 50L units or one of each unit.
CellMaker Plus Controller The Plus Controller allows for additional pH and dissolved oxygen monitoring and automatic control.
CellMaker Low Flow Controller CellMaker Low Flow Controller offers very low air flow which is gentle for cells, and also provides pH and dissolved oxygen monitoring and automatic control.
CellMaker Enclosure The 8L CellMaker Enclosure for a benchtop bioreactor allows a working volume of 1.5L to 8L. There is also an option with 50L Enclosure, allowing working volumes between 10L to 50L.
CellMaker Bioreactor Bags Disposable CellMaker Bioreactor Bags are easily replaced in minutes for a fast turnaround.

CellMaker Comparison and Specification

Features Regular Controller Plus Controller Low Flow Controller
Enclosures      
8L
50L
Bioreactor Bags      
Regular Bioreactor Bag 4L
Regular Bioreactor Bag 8L
Regular Bioreactor Bag 50L
Plus Bioreactor Bag 4L ×
Plus Bioreactor Bag 8L ×
Plus Bioreactor Bag 50L ×
Functional Parameters      
Temperature monitoring and control
Air flow control (built-in air pump; 0.2 - 2.5 LPM) × ×
Air flow control (built-in air pump; 1 - 10 LPM) ×
O2 flow control
Pressure monitoring
pH monitoring and control ×
Dissolved oxygen monitoring and control ×
CO2 flow control ×
N2 flow control ×

Applications

CellMaker airlift bioreactors are ideal for cell culture and fermentation in various applications, including phage production, protein expression, viral vector production, fungal biomass, monoclonal antibodies, stem cells and other biotherapeutics. The single-use bioreactors have been proven successful for microbial fermentations, such as E. coli expression systems, phage amplification, CHO cell culture and antibody production in both GMP and GLP environments.

Microbial Culture

  • Bacteria

The CellMaker Plus system enables excellent mixing and aeration suitable for both oxygen hungry and anaerobic bacterial culture processes. Using CellMaker is very effective in culturing bacteria for growth and reduces downtime and required cleaning.

  • Yeast

The CellMaker Plus is also efficient at growing yeast and proven on strains, such as P. pastoris and brewer’s yeast.

  • Microalgae

The CellMaker Plus system can be customized to run a photo-dependent fermentation allowing for the growth of microalgae cells.

Bacteriophage Amplification

The CellMaker bioreactor systems are designed based on a patented airlift technology to accelerate and simplify the bacteriophage amplification process and increase the level of amplification. CellMaker has been successfully used to produce bacteriophages from Escherichia coli, Salmonella, Listeria, Pseudomonas aeruginosa, Staphylococcus aureus, and Yersinia ruckeri.

  • Automation
  • Scale-up production
  • Quick and easy to use
  • Excellent titers
  • Environmental control
  • Volume efficiency
  • No cleaning or autoclaving required
  • Contamination reduction

Automated Culture of Mammalian Cells

Mammalian cell culture requires precise control of various parameters, accompanied by continuous motion. CellMaker Plus airlift bioreactors with specially designed mammalian disposable bioreactor bags are suited for cell culture. The CellMaker systems provide gentle mixing at low shear stress to produce quality protein and maintains optimal conditions for mammalian cell production throughout the run.

The CellMaker system automates the process of cell culture and produces repeatable results at the touch of a button. Temperature, pH and dissolved oxygen are easily monitored and controlled with built-in probes, and visual inspections are quickly performed through a transparent front panel.

Rapid Production of Quality Antibodies

The production of recombinant antibodies is a labor-intensive process with a high risk of contamination. Due to the use of sterile and disposable bioreactor bags, CellMaker systems allows for fast turnaround with minimal downtime and no risk of contamination. CellMaker has been used for recombinant antibody expression in CHO cells and has also been used to grow hybridoma cell lines for the production of monoclonal antibodies.


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