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•  ZiXpress Magnetic Pillar Nucleic Acid Purification Instruments

Amerigo Scientific offers ZiXpress magnetic pillar nucleic acid purification systems for the purification of nucleic acids from a variety of biological samples. ZiXpress is an open automated nucleic acid extraction platform based on our advanced magnetic beads ZiBeads®, which works efficiently and provides reproducible results for a variety of studies. This reliable platform provides optimized prefilled reagent kit / non-prefilled consumables for free operation. With the possibility of editable protocols, ZiXpress can be used in laboratories in any industry. These systems can be broadly used in many molecular biology downstream applications such as genetic screening, sequencing, food safety, etc.

Specifications of ZiXpress Systems

Models	ZiXpress 32	ZiXpress 64	ZiXpress 96s
Dimensions	36 cm L x 44 cm W x 48 cm H	36 cm L x 72 cm W x 49 cm H	49 cm L x 51 cm W x 48 cm H
Weight	25 kg	38kg	45kg
Throughput	32 tests/run	64 tests/run	96 tests/run
Power Supply	AC 100-240V, 50/60 Hz 400VA, UPS	AC 100-240V, 50/60 Hz 500VA, UPS	AC 100-240V, 50/60 Hz 600VA, UPS
Mixing Mode	Oscillation		Rotation
Display	7 inch color Touch Panel
Sample Volume	200 µl
Elution Volume	100-1000 µl		30-1000 µl
Processing Time	10-42 min		22-60 min
UV Decontamination	√	√	√
Door Sensor	√	√	√
Bottom Touch	√	√	/
Appearance

Oscillatory Mixing Technology of ZiXpress 32/64

The comb of ZiXpress 32/64 is accurately in contact with the bottom of the well in every mixing move step, resulting in efficient mixing and high quality extraction performance of the system.

• Superior efficiency of mixing magnetic beads
• Preventing magnetic beads from gathering
• Consistent and high-quality output of each well

Rotary Mixing Technology of ZiXpress 96s

The rotary mixing technology of ZiXpress 96s overcomes the shortcomings of traditional nucleic acid extraction methods and enables the rapid and efficient preparation of nucleic acids from a large number of samples. The 360-degree rotation produces full-angle oscillations, resulting in faster and more uniform mixing of the magnetic beads with the sample reagent compared to the conventional up-and-down mixers, further ensuring stable and efficient nucleic acid purification.

Figure 1. Mixing Technologies of ZiXpress

Applications of ZiXpress Systems

Compatible ZiXpress DNA/RNA Extraction Kits

Used with our companion kit, the ZiXpress system can rapidly extract nucleic acids from different samples such as whole blood, serum, plasma, swab samples, secretions, culture suspensions, and tissues. Nucleic acid extraction of 32/64 samples can be completed in 10 to 42 minutes by ZiXpress 32/64, which can significantly shorten the overall assay time depending on the protocol.

Performance Data of ZiXpress Systems and Kits

DNA was purified from three different weights of skeletal muscle from 12.5 to 40 mg using the ZiXpress instrument and ZiXpress Tissue gDNA Extraction Kit. The concentration of extracted DNA was measured by spectrophotometer (Fig.2). The range of DNA yield of the ZiXpress Tissue gDNA Extraction Kit is 9 to 36 µg.

Figure 2. Tissue DNA concentration measured by NanoDrop® 2000 spectrophotometer

DNA was purified from 16 duplicate human whole blood samples (in EDTA-K2 collection tube) using the ZiXpress Purification Instrument and ZiXpress Whole Blood gDNA extraction Kit. The concentration of purified DNA was measured using a spectrophotometer (Fig.3). The yield range of the ZiXpress® Whole Blood gDNA Extraction Kit is 1.4 to 8.7 μg DNA from the blood sample with WBCs counts of 1.8 - 10.8 x 10³ cells/ml.

Figure 3. Whole blood DNA concentration measured by NanoDrop® 2000 spectrophotometer