FuGENE® DNA and SiRNA Transfection Reagents
Transfection is a process by which exogenous nucleic acids are delivered into cells to modify the genetic composition of host cells. It is widely used in the study of cellular processes and molecular mechanisms of diseases and in the development of gene therapies. Different types of nucleic acids, including deoxyribonucleic acids (DNAs), ribonucleic acids (RNAs) and small non-coding RNAs (such as siRNA, shRNA and miRNA), can be transfected into mammalian cells by current transfection techniques.
Using liposomal or multicomponent transfection reagents is a well-established transfection method for delivery of nucleic acids into mammalian cell lines. This approach has been widely used for overexpression of proteins with expression plasmids or for specific gene knockdown using siRNA. However, many transfection reagents exhibit toxic side effects, such as reduced cell viability, and these side effects may lead to misinterpretation of the results. Therefore, selection of suitable transfection reagents is crucial to achieve valuable results with minimal side effects.
Amerigo Scientific offers FuGENE® DNA and RNA transfection reagents that are synthetic, multi-component and have extremely low cytotoxicity, high transfection efficiency, few nonspecific side effects with minimal alteration in gene expression. FuGENE® can be used in the presence or absence of serum, and their minimal cytotoxicity eliminates the need for medium changes after transfection. Compared with other methods, transfection of nucleic acids into mammalian cells using FuGENE® can achieve more consistent results, save time and effort, and has minimal impact on cell physiology.
FuGENE® DNA Transfection
Delivery of DNA of all sizes into eukaryotic and insect cells requires transfection reagents that result in robust gene expression of the delivered construct with minimal-to-no cytotoxicity. Amerigo Scientific offers multi-component, non-liposomal or lipid-based transfection reagents with highly efficient transfection and extremely low cytotoxicity to meet the needs of diverse research, such as protein/antibody production, virus production, or breakthrough therapeutic development. Our FuGENE® DNA transfection reagents are suitable for both routine cost-effective transfection and transfection of sensitive, difficult-to-transfect primary or stem cells.
| Product Name |
Description |
Size |
| FuGENE® HD DNA Transfection |
A 100% synthetic, multi-component, non-liposomal transfection reagent designed for the delivery of DNA into eukaryotic and insect cell lines |
1 mL - 50 mL |
| FuGENE® 4K DNA Transfection |
An improved transfection reagent for FuGENE® HD, designed for the delivery of DNA into mammalian cell lines |
1 mL - 50 mL |
| FuGENE® 6 DNA Transfection |
A multi-component lipid-based transfection reagent designed for the efficient delivery of DNA of all sizes into a broad range of cell types |
1 mL - 50 mL |
Key Applications
- Virus and Protein Production
- Gene Expression
- Cellular Analysis
- Cancer Research
- Difficult-to-transfect Cells
- Stable Cell Production
Benefits
- High transfection efficiency in many cell types, including HeLa, NIH-3T3, COS-7, SKOV3, CHO-K1, and many more
- Extremely low cytotoxicity, allowing fewer cells to be used, and eliminating the requirement to change media after the addition of transfection complex
- Suitable for transient and stable transfection
- Exceptionally good functionality in the presence or absence of serum
- Requiring minimal optimization
Easy Optimization for High Performance Transfection
A549, COS7, HCT116, HEK293, and HeLa cells were seeded in 96-well plates and transfected with FuGENE® HD reagents (Left) or FuGENE® 6 reagents (Right) and GFP expressing plasmids in different ratios of FuGENE® reagents to total DNA. Transfection efficiency were analyzed by flow cytometry after 24 hours.
A. COS-7 Cell Line Transfected with FuGENE® HD & GFP Plasmid
B. HEK293 Cell Line Transfected with FuGENE® HD (X-Gal Stain)
C. HEK-293T Viral Production Cell Line Transfected with FuGENE® HD & RFP Plasmid
FuGENE® RNA Transfection
Amerigo Scientific offers FuGENE® RNA transfection reagents for high-efficiency delivery of siRNAs into all eukaryotic cells. FuGENE®SI developed by proprietary chemistry can achieve superior transfection of siRNA or RNA-like molecules to yield maximum gene knockdown with extremely low cytotoxicity.
| Product Name |
Description |
Size |
| FuGENE® SI RNA Transfection |
A 100% synthetic, non-toxic, multi-component transfection reagent designed for high-efficiency delivery of RNA molecules into eukaryotic cell lines |
1 mL - 50 mL |
Application
FuGENE® SI Transfection Reagent is a multi-component reagent that forms complexes with siRNA, miRNA, and other small RNAs. The complexes are then safely and efficiently transported to eukaryotic cells.
Benefits
- Excellent knockdown efficiency while being gentle to cells
- High transfection efficiency in many common cell types and difficult-to-transfect cell lines
- Minimal-to-no cytotoxicity, allowing fewer cells to be used, and eliminating the requirement to change the media
- Requiring minimal optimization
- Flexible and quick protocols
Gentle and Non-Toxic RNA Transfection
HEK293-GFP and NIH3T3-GFP Cell lines were seeded in 96-well plates and subsequently transfected with 0.2, 1.0 or 5 pmols of GFP targeting siRNA or negative control, along with 0.3 μl of FuGENE® SI transfection reagents. Total cell viability was measured by flow cytometry 48 h after transfection, indicating that gentle and nontoxic transfection of siRNA could be achieved using FuGENE® SI.
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