• Amerigo Scientific Instrument
  • Polysaccharide-based chiral stationary phases (CSPs) are one of the most commonly used CSPs in the chiral high performance liquid chromatography (HPLC) and show excellent performance in analytical and preparative chiral separation. The chiral selectivity of polysaccharide derivatives depends on different their structural features, including the sugar unit, the stereogenic centers of the glucopyranose units, the type and position of the linkage, and the adjacent polymer chains.

    Polysaccharide derivatives can be coated or immobilized on chromatographic supports. CSPs comprising coated polysaccharide derivatives are intolerant to many solvents such as dichloromethane, chloroform, toluene, or acetone, which results in limited application. The use of these solvents in the mobile phase may lead to the dissolution of the adsorbed polymer. CSPs comprising immobilized polysaccharide derivatives are a reliable alternative, allowing the use of a wider selection of solvents as the mobile phases. The immobilization step cross-links the polysaccharide backbone and bonds it to the silica gel surface, thereby increasing CSPs robustness and solvent compatibility.

    Immobilized Polysaccharide CSPs

    The feature of immobilized polysaccharide CSPs combines the wide applicability and high loading capacity of the coated polysaccharide phase with universal solvent compatibility, making the chiral columns based on immobilized polysaccharide CSPs an effective tool for chiral separation. The immobilized polysaccharide stationary phases show stability to the pressure up to 400 bar. The high stability ensures efficient column operation over an extended period of time. In addition, the immobilized CSPs have high complementarity for the separation of chiral compounds.

    Product Range

    Amerigo Scientific offers chiral HPLC columns based on immobilized polysaccharide CSPs. Immobilization of the polysaccharide derivatives on the silica gel support leads to the universal solvent compatibility of CSPs. Most miscible organic solvent can be used with our immobilized type chiral HPLC columns, enabling new selectivity profiles and improved productivity.

    Product Name Chiral Selector Particle Size
    CHIRALPAK® IA / IA-3 / IA-U Amylose-tris (3,5-dimethylphenylcarbamate) 1.6 μm, 3 μm, 5 μm
    CHIRALPAK® IB / IB-3 / IB-U Cellulose-tris(3,5-dimethylphenylcarbamate) 1.6 μm, 3 μm, 5 μm
    CHIRALPAK® IB N-5 / IB N-3 Cellulose-tris(3,5-dimethylphenylcarbamate) 3 μm, 5 μm
    CHIRALPAK® IC / IC-3 / IC-U Cellulose-tris(3,5-dichlorophenylcarbamate) 1.6 μm, 3 μm, 5 μm
    CHIRALPAK® ID / ID-3 / ID-U Amylose tris(3-chlorophenylcarbamate) 1.6 μm, 3 μm, 5 μm
    CHIRALPAK® IE / IE-3 Amylose tris(3,5-dichlorophenylcarbamate) 3 μm, 5 μm
    CHIRALPAK® IF / IF-3 Amylose tris(3-chloro-4-methylphenylcarbamate) 3 μm, 5 μm
    CHIRALPAK® IG / IG-3 / IG-U Amylose tris (3-chloro-5-methylphenylcarbamate) 1.6 μm, 3 μm, 5 μm
    CHIRALPAK® IH / IH-3 / IH-U Amylose tris[(S)-α-methylbenzylcarbamate] 1.6 μm, 3 μm, 5 μm
    CHIRALPAK® IJ / IJ-3 Cellulose tris(4-methylbenzoate) 3 μm, 5 μm
    CHIRALPAK® IK / IK-3 Cellulose tris (3-chloro-5-methylphenylcarbamate) 3 μm, 5 μm
    CHIRALPAK® IM / IM-3 Cellulose tris(3-chloro-4-methylphenylcarbamate) 3 μm, 5 μm
    CHIRALPAK® IN / IN-3 Amylose tris(5-chloro-2-methylphenylcarbamate) 3 μm, 5 μm

    Features

    Our chiral HPLC columns packed with immobilized polysaccharide CSPs have outstanding solvent tolerance, which are designed for the separation of chiral compounds with limited solubility and show excellent performance in enantiomer separation.

    • High separation efficiency
    • Compatible with organic solvents as mobile phases
    • High possibility for successful baseline separation
    • Simple work for sample separation

    Operating Conditions

    Limit of Pressure 330Mpa for columns packed with 3μm /5μm CSPs;
    70Mpa for columns packed with 1.6μm CSPs
    pH Range pH 2.0 - 9.0
    Temperature 0°C - 40°C
    5°C - 25°C (Reverse phase, pH>7)

    Mobile Phase

    Various organic solvents can be used as mobile phases or to dissolve samples, such as:

    • N-hexane
    • 2-propanol
    • Methyl tert-butyl ether (MTBE)
    • Dichloromethane
    • Tetrahydrofuran
    • Ethyl acetate
    • Dimethyl sulfoxide (DMSO)
    • Trichloromethane
    • Acetonitrile
    • Methylbenzene
    • 1,4-dioxane
    • H₂O

    Please note the miscibility between solvents.


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