3D Electrospun Polycaprolactone Scaffolds
Electrospinning is an effective and versatile method to process polymer solution or melt, mainly of polymers, into continuous micro-/nano-fibers in high electric fields. The advantage of electrospinning is that almost all polymers and drugs can be electrospun into tunable and uniform nanofibers. In addition, composite nanofibers can also be easily developed by blending macromolecule spinning solutions via electrospinning.
Polycaprolactone (PCL) is a biodegradable polymer with good biocompatibility, low cost, and easy processability, which is approved by the US Food and Drug Administration (FDA) for biomedical applications. Nanofibers prepared by electrospinning from biocompatible and biodegradable polymers have highly similar properties to the extracellular matrix (ECM), with high cell viability and biocompatibility. PCL electrospun nanofibers are a cost-effective and ECM-like cell supports for 3D cell culture.
Electrospun Fiber Meshes for 3D Cell Culture
Amerigo Scientific offers polycaprolactone fiber products produced by advanced electrospinning processes, including nanofiber and microfiber bi-layer mesh products as well as conventional nanofibers and microfibers. Compared with traditional products, our electrospun fiber mesh products have higher rigidity with high ease of use.
| Product Name |
Description |
| BILAMINATE
|
Layered nano- and microfibers providing authentic beneficial effects to 3D cell culture |
| MicroFIBERS
|
Microfibers with large pore size and high rigidity that can be used as hydrogel support materials |
| NanoFIBERS
|
Traditional nanofiber meshes with high quality consistency providing structural support for cell adhesion and growth |
Specifications
| |
BILAMINATE |
MicroFIBERS |
NanoFIBERS |
| Dimension (mm²) |
About 84 × 84 |
| Materials |
Polycaprolactone (PCL) |
| Sterilization / surface treatment |
UV (option: non-sterile or O₂-plasma treatment) |
| Fiber type / average thickness (μm) |
Microfibers (unaligned) : 40 ± 10 or 60 ± 15
Nanofibers : 30 ± 7.5 (unaligned) or 12 ± 3 (aligned) |
unaligned : 60 ± 15 |
Unaligned : 30 ± 7.5 or 60 ± 15 |
| Average fiber diameter |
Microfibers : 5.7 ± 1.2 μm
Nanofibers : 770 ± 77 nm |
5.7 ± 1.2 μm |
770 ± 77 nm |
| Microscopic structure |
 |
 |
 |
Applications
- 3D cell culture of a variety of cell types
- In vitro experiments in tissue engineering and cell therapy
- Various biochip applications
Our products are attached to aluminum foil. Before use, please cut the product into specific size pieces as needed, and then separate the product from the aluminum foil.
Electrospun Fibers with A 3D-Printed Frame
Amerigo Scientific offers electrospun fiber products with a nanofiber-frame combination structure, which overcome the limitations of nanofibers including low stiffness and agglomeration when wet. These products are available in various electrospun fiber arrangements, diameters, thicknesses, and frame patterns.
| Product Name |
Description |
|
FrameSHEET
|
A frame-fiber product that can be cut and used depending on experimental requirements |
|
FiberDISK
|
A disk-shaped fiber product designed to correspond to standard cell culture well plates |
Specifications
| |
FrameSHEET |
FiberDISK |
| Frame pattern |
Lattice |
Circles/Lattice |
Average
dimension |
Outer size (mm²) |
About 80 × 80 |
6, 10 or 15 |
| Height (μm) |
200 ± 40 |
380 ± 50 |
| Fiber type /average thickness (μm) |
Microfibers: 18 ± 4.5 (unaligned) or 18 ± 4.5 (aligned)
Nanofibers: 30 ± 7.5 or 60 ± 15 (unaligned) or 12 ± 3 (aligned) |
| Average fiber diameter |
Microfibers: 5.7 ± 1.2 μm
Nanofibers: 770 ± 77 nm |
| Fiber materials |
Polycaprolactone (PCL) |
| Average frame width (μm) |
385 ± 75 and 435 ± 75 |
385 ± 75 |
| Frame materials |
Polycaprolactone (PCL) |
| Sterilization / surface treatment |
UV and O2-plasma treatment (other options: non-sterile or UV only) |
| Shape |
 |
 |
Features
- Authentic combination of 3D printing frame and nanofibers
- Fully free-standing ultra-thin nanofiber layers
- Nanofiber meshes with thickness ranging from a few to tens of micrometers
- High transmittance and good observation performance
- Easy to observe adhesion/growth behavior of cells on nanofibers
Applications
- Porous support for cell-laden hydrogel
- 3D cell culture of a variety of cell types
- In vitro experiments in tissue engineering and cell therapy
C2C12 cells and NIH3T3 cells cultured on PCL nanofibers
Frequently Asked Questions
What types of cell assays can your electrospun polycaprolactone products be used for?
To date, cells representing all basic tissues have been successfully cultured, and experiments with more cell types are ongoing.
What material is your products made of?
Our product is made of polycaprolactone (PCL), a biodegradable polymer.
Can these products be reused?
No. All products are disposable.
Are these products packaged under sterile conditions?
Yes. All products are sterile and ready to use.
How to store the remaining products after use?
Please put the remaining products into the box and store them at low temperature (refrigerator, freezer, quick freezer).
* When using the remaining products stored at low temperature, pay attention to moisture.
Are these products chemically stable?
Yes. PCL is a very stable material. However, some chemicals such as acetone or xylene may cause fibrous deformation.
What methods can be used to sterilize these products?
All products can be sterilized with UV. If ethanol is used to sterilize these products, see the sterilization manual.
* Sterilization can be carried out according to the user's own protocol, but not by autoclaving.
Should the remaining fibers be sterilized separately before use?
No. These products have already been sterilized for sale, and since ethanol is used to separate fibers and has a sterilization effect, no other sterilization is required.
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