• Amerigo Scientific Instrument
  • Cell cryopreservation is a technique that allows cells to maintain their cellular viability and function at low temperatures, usually -80 °C or -196 °C. In addition, cryopreservation prevents cells from accidental mutation, senescence, and contamination. With the development of stem cell therapy, tissue engineering, assisted human reproduction and transfusion medicine, cell cryopreservation has become an important supporting technology for these cell-based applications. During the freeze-thaw cycle, cells inevitably suffer cryoinjuries, including solution injury and ice injury. Cryoprotectants (CPAs) play a key role in protecting cells from these cryoinjuries. CPAs can be classified into two major groups based on their permeability or nonpermeability to cell membranes. Permeating CPAs mainly include organic solvents such as glycerol and dimethyl sulfoxide (DMSO). Fetal bovine serum (FBS) is often combined with organic solvents to supplement extracellular cryopreservation. However, the use of FBS presents a potential direct path of cell culture contamination and carries a risk of inducing viral infection and immunogenicity. Serum-free media alleviate the difficulties caused by the availability and cost of FBS. The risk of exposure to harmful diseases can be reduced by using serum-free media for long-term storage of cells.

    GMP-grade Cell Freezing Media

    Amerigo Scientific offers a series of GMP-grade, ready-to-use, serum-free cell freezing media for cryopreservation of cell lines, primary cells, stem cells, or immune cells. The GMP-grade cell freezing media are available in formats containing 10% DMSO or without DMSO. The use of these media simplifies the freezing process and greatly increases cell viability.

    Features

    • Ready-to-use and chemical defined medium
    • Serum-free, Xeno-free, animal-derived component-free
    • Manufactured in GMP-compliant facilities
    • No programmed or sequential freezing required
    • Sterile, low endotoxin (<0.125 EU/mL)
    • High recovery rate

    Product Range

    • Universal Cell Freezing Media
    Product Name Type Application
    Cell Cryopreservation Medium containing 10% DMSO for various cell lines and primary cells
    Cell Cryopreservation Medium Plus DMSO-free
    Cell Cryopreservation Medium Plus containing 10% DMSO

    • Stem Cell Freezing Media
    Product Name Type Application
    iPSC Cryopreservation Medium containing 10% DMSO for iPSCs from various sources such as blood cells, epithelial cells, fibroblasts, etc.
    Stem Cell Cryopreservation Medium DMSO-free for mesenchymal stem cells from various sources such as umbilical cord mesenchymal stem cells and bone marrow mesenchymal stem cells
    Stem Cell Cryopreservation Medium containing 10% DMSO

    • Immune Cell Freezing Media
    Product Name Type Application
    αβ-T Cell Cryopreservation Medium DMSO-free for αβ-T cells
    αβ-T Cell Cryopreservation Medium containing 10% DMSO
    γδ-T Cell Cryopreservation Medium containing 10% DMSO for γδ-T cells
    NK Cell Cryopreservation Medium containing 10% DMSO for NK cells
    PBMC Cryopreservation Medium DMSO-free for mononuclear cells from peripheral blood and umbilical cord blood
    PBMC Cryopreservation Medium containing 10% DMSO

    Application Examples

    Four types of cells were cryopreserved using our GMP-grade Cell Cryopreservation Medium. Figures 1-4 sequentially show the morphology of Vro cells, HEK293 cells, NH3T3 cells, and Jurkat cells on the third day after resuscitation.

    Cell Cryopreservation Medium

    The iPSCs that had been cryopreserved using our GMP-grade iPSC Cryopreservation Medium showed good adherent growth after resuscitation. Immunofluorescence staining for OCT3/4, NANOG, TRA-1-60, and SSEA-4 confirmed the expression of canonical pluripotency markers in the iPSCs at 72 hours after resuscitation.

    iPSC Cryopreservation Medium

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