BcMag™ NHS-activated Magnetic Beads Conjugation Buffer Kit

BcMag™ NHS-Activated Magnetic Beads are uniform, silica-based superparamagnetic beads coated with high density NHS (N- hydroxyl succinimide) functional groups on the surface. The beads are used to specifically conjugate primary Amine-containing ligands, so other active groups in the ligand do not need to be protected prior to coupling. Coupling is fast (15–30 min at room temp pH 6.5–9, 4 hours at 4 °C). BcMag™ NHS-activated Magnetic Beads are most suitable for conjugation of large proteins. BcMag™ Long-arm (17 atome) NHS-activated Magnetic Beads are recommended for conjugation of small peptides because the long-arm (21-atom) hydrophilic linker may reduce steric hindrance.

• Pre-activated and ready-to-use
• Quick coupling in neutral pH (6.5–9) 4 to 25°C.
• Stable covalent bond with minimal ligand leakage
• Produces reusable immunoaffinity matrices
• Low nonspecific binding
• Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads
• Applications: Cell sorting, Immunoprecipitation; Purification for Antibodies, Proteins/Peptides, DNA/RNA

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