MagCore® Automated Nucleic Acid Extraction

MagCore® nucleic acid extraction kits contain all the required reagents and consumables for rapid, automated, and contamination-free purification of DNA and RNA from specific samples using magnetic particle technology. The reagents are supplied in pre-filled and pre-sealed reagent cartridges, which ensures easy and fast instrument loading procedures. No additional reagents are required, and no manual processing is required. Sealed cartridges and automated piercing steps eliminate the risk of contamination and spillage. Mixing of MagCore® patented beads, reagents and sample is performed by the liquid handling function of the MagCore® instrument. The nucleic acid separation and high-yield isolation are achieved through magnetic attraction and specific liquid handling motion.

Amerigo Scientific offers a wide range of MagCore® nucleic acid purification kits for the purification of DNA or RNA from specific samples, which are specially designed to be employed in MagCore® Super extractor (1-16 samples per run) and MagCore® Plus II extractor (1-16 samples per run).

MagCore® Magnetic Beads

MagCore® patented magnetic beads are internally multicore, cellulose-coated magnetic beads. The core material is Fe3O4. MagCore® magnetic beads can bind to the specific DNA or RNA fragments with high affinity, ensuring that all important genetic information is collected efficiently, making daily isolation routine easier and faster.

MagCore® Cartridge

MagCore® nucleic acid extraction kits contain all reagents and consumables needed for 36, 72 or 96 nucleic acid isolation reactions. The consumables consist of reagent cartridges, individually packaged tip sets, sample tubes and elution tubes. The patented Heating Well and Separation Well in the sealed cartridge provide a strong circular force to ensure efficient binding and washing during the extraction procedure.

Extraction Principle

MagCore® Nucleic Acid Extraction Kits

• Kits for the extraction of genomic DNA from human whole blood sample

MagCore® genomic DNA whole blood kits are designed for extraction of total DNA (including genomic, mitochondrial, and viral DNA) from whole blood, plasma, serum, and buffy coat. The pre-filled cartridge contains proteinase K, chaotropic salt, and guanidine hydrochloride for cell lysis and protein degradation, which enhances the binding between cellulose-coated magnetic beads and DNA. High quality DNA is eluted by low salt elution buffer or water after the removal of contaminants. Purified DNA of approximately 20-30 kb is suitable for PCR or other downstream applications.

• Kits for the extraction of genomic DNA from other samples

• Kits for the extraction of free circulating DNA from human plasma or serum

MagCore® Plasma DNA Extraction Kit and MagCore® Circulating DNA Large Volume Kit are designed for purifying free circulating DNA from human serum or plasma. The purified high-quality DNA can be used for various downstream applications such as PCR, real-time PCR, and next generation sequencing (NGS).

• Kits for the extraction of viral DNA/RNA from serum, plasma, and cell-free body fluids

MagCore® viral nucleic acid extraction kits are designed for purifying viral DNA and RNA from serum, plasma, and cell free body fluids. The purified high-quality DNA/RNA is suitable for direct use in downstream applications such as amplification or other enzymatic reactions. All plastic consumables inside the kit are DNase/RNase treated to minimize the risk of cross contamination. Multiple protocols are installed on MagCore® automated nucleic acid instruments and optimized for different sample volumes.

• Kits for the extraction of total RNA from whole blood, cultured cells, or FFPE tissues

Amerigo Scientific offers different kits for the purification of total RNA from different samples. MagCore® Total RNA FFPE One-Step Kit is specially designed for the purification of total RNA from formalin-fixed paraffin-embedded (FFPE) tissues by MagCore® instruments. The product is characterized by a one-step heating method to melt paraffin in the absence of harmful reagents such as xylene or other organic solvents while lysing the tissue. The MagCore® Total RNA FFPE One-Step Kit System optimizes the lysis conditions to reverse the formalin fixation without the need for overnight digestion and retain both large and small RNAs. The program provides optional DNase I treatment to remove contaminated DNA.

MagCore® Automated Nucleic Acid Extractors

MagCore® Extractors are simple, fast, and cost-effective instruments for automated purification of nucleic acids from a diverse range of sample sources. Featuring preprogrammed protocols and a unique magnetic bead technology, MagCore® systems provide efficient and consistent nucleic acid purification. Amerigo Scientific offers MagCore® extractors that are benchtop instruments and ensure efficient and cross-contamination-free DNA/RNA isolation. Built-in UV lamps allow easy and effective disinfection of the instrument after operation.

MagCore® Automated Nucleic Acid Extractors	MagCore® Plus II	MagCore® Super
Instrument Shape
1- 16 Samples	⏹	⏹
Spectrophotometer		⏹
Touch Screen	⏹	⏹
UV Decontamination	⏹	⏹
Barcode Scanner	⏹	⏹
Thermal Printer		⏹
Built-in Programs (Upgradeable via USB ports, Plug&Play)	⏹	⏹
USB Output (USB flash drive not provided)	⏹	⏹
Progress Monitoring (Wireless)	⏹
LIMS (Laboratory Information Management System)	⏹	☐

⏹ Standard ☐ Optional

Flexibility

MagCore® automated extraction system saves time without sacrificing consistency and purity. One MagCore® instrument can be used to purify DNA and RNA from a broad variety of sample types, from blood to mouse tails and almost everything in between. We offer MagCore® kits designed for blood, plasma, cell, tissue, FFPE tissue and plant samples, to fit all your research needs.

Ease of Use

Amerigo Scientific offers everything you need to run purifications, including pre-filled cartridges, specialized disposable tips and tubes. The user-friendly interface and user manuals ensure you easy operation.

Safety

MagCore® automated extraction system helps to minimize cross-contamination by limiting operating procedures and turnaround times. The MagCore® system accelerates front-end processing, enabling more tests in less time. The MagCore® instrument is compact enough to virtually fit into almost any laboratory.

Built-in Programs

MagCore® Extractor models have built-in protocols for all the kits we offer. Simply run the protocol by selecting the 3-digit code printed on the kit of interest. Free upgrade of software and protocols can be downloaded and uploaded through the instrument RS232 / USB ports.