Human platelet lysate (HPL) is a multifactorial supplement obtained from human platelets, which contains abundant growth factors and cytokines necessary for cell growth and proliferation. In a wide range of applications, HPL is considered a good substitute for animal serum. The replacement of fetal bovine serum (FBS) with HPL can reduce the immunogenicity and remove the transmission of zoonotic diseases, which is beneficial to the clinical application of cell proliferation or production.
Two main differences in HPL protein content compared to FBS are the higher content of immunoglobulins and the possible presence of fibrinogen and other coagulation factors, when HPL is produced without thrombin activation.
Mean Protein Content in HPL and FBS | ||||||
---|---|---|---|---|---|---|
Total Protein | Albumin | Immunoglobulin G | Immunoglobulin A | Immunoglobulin M | Fibrinogen | |
HPL* | 55-65 mg/mL | 35-40 mg/L | 8-12 mg/mL | 1-2 mg/mL | 0.5-1.5 mg/ mL | 1.0-3 mg/mL** |
FBS | 2-70 mg/mL | 20-36 mg/mL | - | - | - | - |
* HPL prepared from a platelet concentrate obtained stabilized in plasma; ** HPL obtained by freeze-thaw or S/D processes
HPL contains high concentrations of growth factors, such as insulin-like growth factor-1 (IGF1), platelet derived growth factor (PDGF)-AA, -BB, -AB, transforming growth factor (TGF)-β1, brain derived neurotrophic factor (BDNF), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and basic fibroblast growth factor (bFGF). The contents of these growth factors range from 0.1 to 300 ng/ml, and TGF-β1, PDGF-AB, IGF1, and BDNF are generally higher in content than other growth factors.
HPL, like FBS, contains minerals such as sodium, potassium, magnesium, iron, as well as glucose and vitamins that can influence cell growth, differentiation, and function.
Amerigo Scientific offers research-grade and GMP-grade HPL products derived from platelets of healthy voluntary donors at certified blood banks. All HPL products are naturally free form other animal derived material. No animal serum is added during the manufacture of these products. Two formats are available according to the need of applications, as follows:
PRODUCT NAME | Description | Applications |
---|---|---|
ELAREM™ Kit Matrix - hPL Gel | a cell culture system based on HPL | 2D / 3D cell culture |
HPL ELAREM™ Perform, heparin required | HPL of EU origin | cell culture |
HPL ELAREM™ Perform FD, no heparin required | a fibrinogen-depleted HPL of US origin | cell culture |
HPL ELAREM™ Prime, heparin required | HPL of EU origin with consistency from batch to batch | cell culture |
ELAREM™ Supplement- Heparin, synthetic, preservative-free | an anticoagulant to be used with fibrinogen-containing HPL | supplements |
PRODUCT NAME | Description | Applications |
---|---|---|
HPL ELAREM™Perform, heparin required | HPL of EU origin | cell culture |
HPL ELAREM™ Perform FD, no heparin required | a fibrinogen-depleted HPL of US origin | cell culture |
HPL ELAREM™ Ultimate FDi, Gamma Irradiated, fibrinogen depleted, no heparin required | a virus-inactivated and fibrinogen-depleted HPL of US origin | cell culture |
ELAREM™ Supplement- Heparin, synthetic, preservative-free | an anticoagulant to be used with fibrinogen-containing HPL | supplements |
References
Human AB serum is an ideal supplement for human derived cell lines compared with other animal sera such as fetal bovine serum (FBS). Amerigo Scientific offers human AB serum collected from healthy volunteer male donors of the AB serotype. The material is CE marked and ANSM approved IVDMD.
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