2'-O-Methyl (Nm) is one of the most common and widespread type of post-transcriptional modification found in both non-coding and coding RNAs, such as ribosomal RNA (rRNA), transfer RNA (tRNA), small nucleolar RNA (snRNA), and messenger RNA (mRNA). Nm modification is critical for cellular activity of many RNAs and misregulation of Nm is involved in diseases. In addition, Nm can increase RNA stability, promote RNA folding and facilitate protein binding in a space-dependent and/or time-dependent ways. Structurally, the addition of Nm modification stabilizes the ribose 3'-endo conformation, which in turn facilitates the formation of an A-type RNA helix. Chemically, Nm changes the hydrogen bonding properties of the 2'-O ribose site and inhibits its nucleophilicity. As a result, Nm makes RNA more stable and less sensitive to alkaline hydrolysis, nuclease cleavage, and oxidation.
2'-O-Methyl RNA can be synthesized directly from oligonucleotides and has unique biological characteristics. As a versatile tool in molecular biology, 2'-O-Methyl oligonucleotides can be used as chimeric oligomers with DNA-oligomers for site directed cleavage of RNA with Ribonuclease H (RNase H). 2'-O-Methyl oligoribonucleotides form stable hetero-duplexes with the complimentary RNA and the melting point (Tm) of the hybrid is higher than that of the corresponding DNA sequence. Additionally, 2'-O-Methyl oligonucleotides are developed for diagnostic and therapeutic products. Amerigo Scientific offers high-quality phosphoramidites (amidites) and supports for the synthesis of 2' & 3' -O-Methyl RNA.
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