Membrane Protein Detergents and Reagents

Most drug discovery programs and structural biology studies require access to isolated, stable, and functional membrane proteins in solution. Solubilizing a membrane protein, while maintaining its stability, is an enduring challenge in membrane protein structural biology. The most efficient method to achieve this is to use surfactants, such as detergents, to allow the membrane proteins to retain all their properties.

From membrane protein extraction, stabilization, isolation to crystallization, we offer membrane protein detergents, reagents, and surfactant kits for your discovery projects to improve the poor solubilization rate and the weak stability of membrane proteins.

Advantages

Our detergents and reagents:

  • enable researchers to work with membrane proteins in a native process without any denaturing steps
  • improve protein stability without mutations, truncations, or modifications
  • isolate native wild-type, full-length, stable, and functional membrane proteins
  • can be used alone or in combination with your favorite detergent
  • are compatible with all commercial detergents

Product Range

  • Soft Proprietary Membrane Protein Detergents

These detergent molecules are designed to structure membrane proteins through hydrophobic interactions. Because these molecules are non-ionic, they are not sensitive to variations in ionic strength or pH and are ideal for extracting, solubilizing, and stabilizing specific membrane proteins.

Product Name Description
CALX8 Reagent an ionic calixarene based detergent/surfactant with three polar carboxylic groups and an octyl chain.
DDG Reagent a non-ionic amphiphilic detergent and surfactant with a di-glucose polar group and decyl chain.
DDTAC Reagent a non-ionic detergent that can be used to solubilize, extract, and stabilize membrane proteins.
DLAC Reagent a non-ionic detergent / surfactant with a lactobionamide head group and a decyl chain.
FLAC6 Reagent a non-ionic amphiphilic detergent / surfactant with a lactobionamide head group and a fluorinated chain.
ODG Reagent a non-ionic amphiphilic detergent / surfactant with a diglucose polar group and an octyl chain.
  • Soft Proprietary Stabilization Reagents

Soft proprietary stabilization reagents are ideal tools for isolating full-length membrane proteins in solution with the highest purity levels, while keeping the structural and functional integrity of membrane proteins. These reagent molecules are non-ionic and thus stabilize specific membrane proteins even in environments where ionic strength or pH fluctuates. Except for DDLAC, other reagents do not dissolve lipid membranes due to their fluorinated chains.

Product Name Description
CALXCHOL Reagent a non-ionic detergent that is primarily used to solubilize and to stabilize membrane proteins (GPCRs, ion channels, transporters).
CALXGLUK Reagent a calixarene detergent that can be used to solubilize membrane protein as GPCRs.
DDLAC Reagent a non-ionic detergent / surfactant with a lactobionamide head group and a dodecyl chain.
FTAC6 Reagent a non-ionic detergent that is primarily used to extract, solubilize, and stabilize GPCRs, ion channels, and transporters.
FTAC8 Reagent a non-ionic detergent that is principally used to extract, solubilize, and stabilize GPCRs, ion channels, and transporters.

Application

Our detergents and reagents are designed to preserve the original structure and function of membrane proteins (GPCRs, ion channels, transporters, receptors, anchors, and viral proteins), providing solutions to develop conformational antibodies, formulate new vaccines, carry out structure-based drug discovery and/or high throughput screening.

  • Antibodies (including nanobodies, scaffold proteins, aptamers)
  • Small molecules
  • 3D structures (cryoEM, XRay crystallography, NMR, SANS, SAXS)
  • Drug discovery (screening and validation)
  • Antibody discovery (immunization and display technologies)
  • Clinical stage


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