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Overview
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BcMag™ Sulfhydryl-Terminated Magnetic Beads are uniform, silica-coated superparamagnetic beads. The Sulfhydryl- Terminated- Magnetic Beads are specifically designed for conjugation of proteins/peptides modified by SMCC (Sulfosuccinimidyl 4-[N-maleimidomethyl]cyclohexane-1-carboxylate, Pierce, cat# 22122). SMCC contains an amine reactive N- hydroxyl succinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react withsulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The procedure is quick and simple for samplepreparation without the need for laborious repeat of pipetting and centrifuging. BcMag™ Sulfhydryl-Terminated Magnetic Beads are most suitable for conjugation of large proteins. BcMag™ Long-arm Sulfhydryl-Terminated Magnetic Beads are recommended for conjugation of small peptides because the long-arm (18-atom) hydrophilic linker may reduce steric hindrance.
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Overview