A Highly Automated Platform for In-depth Proteomics Research
Amerigo Scientific offers proprietary technology-based Proteonano™ Ultraplex Proteomics Platform, specifically designed for large-scale proteomics research to facilitate biomarker discovery or precision medicine development. This platform is a highly automated and standardized system consisting of the protein enrichment kits, high-throughput sample preparation workstation, and AI-powered proteomics analysis software to overcome the challenge of detecting low-abundance proteins in mass spectrometry-based proteomics.
Both G1 Basic and G1 Pro are equipped with an 8-channel pipette, capable of detecting a minimum of 8 samples per run. The basic modules include the magnetic module, gripper module, and heater shaker module. Compared to G1 Basic, G1 Pro adds the SPE desalting module and UV sterilization module, as well as the automatic information input and customized protocol programming functions.
Proteonano™ Proteomics Enrichment Kits
The Proteonano™ kit contains AI-designed polypeptides to selectively bind and enrich low-abundance proteins in biofluid samples with picogram sensitivity and peptide-level specificity.
In the Proteonano™ proteomics enrichment kit, proprietary designed peptides can selectively bind low abundance proteins in biological fluid samples with high affinity. The peptides are attached to superparamagnetic nanoparticles with a diameter of only 200 nm that are homogeneous. Thus, these functionalized nanoscale protein binders have about 300,000 protein binding sites per particle, which enables them to bind and enrich thousands of proteins in an extremely small space.
The volume around each nanoprobe is only 10 fL, which is 10 billion times smaller than conventional immunological methods. This large reduction in volume elevates the sensitivity of binding to low-abundance proteins by one million times. Thus, Proteonano™ nanoprobes can be used to enrich low abundance proteins, post-translational modifications (PTMs), and isoforms that cannot be detected by conventional mass spectrometry. Proteonano™ technology enables the analysis of low-abundant proteins in biological samples at unprecedented depth and throughput, making it a powerful tool for large-scale protein biomarker discovery.
Proteonano™ Proteomics Platform Workflow
The Proteonano™ proteomics platform is compatible to different types of high-resolution mass spectrometers such as Orbitrap Exploris™ 480, Orbitrap Astral™, and timsTOF Pro 2.
Advantages of Proteonano™ Proteomics Platform
This highly automated, high-throughput proteomics platform:
Enables up to 6,000 protein detection simultaneously in a single LC-MS measurement.
Requires only 20 μL of plasma, serum, saliva, or a variety of other types of samples.
Achieves robustness and reproducibility with a median CV lower than 15%.
Identifies a greater variety of proteins and prevents the loss of valuable protein information, increasing the number of proteins detected, compared with the depletion method.
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