-
-
Overview
-
BcMag™ Epoxy-Activated Magnetic Beads are pre-activated ,uniform, silica-based superparamagnetic beads coated with high density epoxy functional groups on the surface. The beads are used to covalently conjugate amine, sulfhydryl, or hydroxyl group-containing ligands. Coupling of Hydroxyl-, Amine- and Thiol-containing ligands is favored at pH 11-12, pH >9, and pH 7.5-8.5, respectively. Water-insoluble ligands can be conjugated in 50% organic solvent (Dioxane, Dimethylformamide). BcMag™ Epoxy-Activated Magnetic Beads are most suitable for conjugation of large proteins. BcMag™ Long-arm Epoxy-Activated Magnetic Beads are recommended for conjugation of small peptides because the long-arm (21- atom) hydrophilic linker may reduce steric hindrance.
• Pre-activated and ready-to-use
• Covalently couples with high efficiency at pH 9–12, 20°C to 40°C, 16 h–days
• Stable covalent bond with minimal ligand leakage
• Produces reusable immunoaffinity matrices
• Low nonspecific binding
• Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads
• Applications: Cell sorting, Immunoprecipitation; Purification for Antibodies, Proteins/Peptides, DNA/RNAPlease contact us at for specific academic pricing.
More Details
-
- Properties
- Applications
-
Overview