Biological buffers and reagents are key tools for life sciences laboratory to obtain accurate and reliable results. Buffers are aqueous systems that resists pH changes when small amounts of acid or base are added. The buffer solution consists of a proton donor (weak acid or base) and its proton acceptor (conjugate base or acid). In a solution with equal concentrations of the proton donor and conjugate proton acceptor, the equilibria of reversible reactions results in buffering.
Almost all biological processes are pH dependent and may be severely affected by even a slight change in pH. The role of buffers in biological systems is to maintain the intracellular and extracellular pH within a narrow range and to resist changes in pH in the presence of internal and external influences. Most buffers used for cell culture, cell separation, enzyme analysis and other applications must have the following characteristics, such as the pKa between 6.0 and 8.0, high water solubility and minimal solubility in organic solvents, no interference with biological processes, non-toxicity, chemical stability, ease-to-use, etc.
Amerigo Scientific offers a wide range of molecular biology grade solutions ideal for a wide range of common applications, such as PBS, TBS, Tris-Glycine, EDTA, TE, TAE, TBE or Tris. Our time-saving solutions are free of toxicity and have great stability, a very precise PH, and showing minimal salt-driven effects. These products are manufactured in accordance with rigorous quality control standards to guarantee lot-to-lot consistency.