BcMag™ Antibody Purification Kits

Amerigo Scientific offers two types of magnetic beads including BcMag™ magnetic beads and EcoMag™ magnetic particles. BcMag™ magnetic beads are uniform, monodispersed, spherical, core-shell superparamagnetic beads which consist of a nanometer-scale superparamagnetic iron oxide core completely encapsulated by a high purity silica shell. EcoMag™ magnetic particles are irregular, silica-coated superparamagnetic particles, which can be used for purification of proteins, nucleic acids, and antibodies. Silica is an excellent separation medium for purification of target molecules due to its superior mechanical and chemical stability.

Features and Advantages of Magnetic Beads

Specifications of Magnetic Beads

Binding Properties of Antibody Binding Proteins

Protein A, Protein G, Protein A/G and Protein L are native and recombinant proteins of microbial origin that bind to mammalian immunoglobulin molecules. These antibody binding proteins are often used in antibody purification, immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP). Protein A and Protein G are expressed in Staphylococcus aureus and Streptococcus sp., respectively, and are used to purify large amounts of IgG. Protein A/G is a fusion protein that combines Protein A and Protein G, which makes it more flexible in antibody purification than Protein A or Protein G alone. Unlike protein A, protein G or protein A/G, protein L binds to kappa light chains (κ chain of the VL domain) of immunoglobulins rather than the Fc region (the heavy chain). Therefore, protein L can bind not only IgG, but also other types of Ig including IgM, IgA, IgE and IgD, as well as single chain variable fragments (scFv) and Fab fragments.

Each antibody binding protein has different binding abilities with different subtypes and types of antibodies, as follows:

++++ (Strong Binding); +++ (Medium Binding); ++ (Weak Binding); - (No Binding); N/A (No Information)

BcMag™ Antibody Purification Beads and Kits

BcMag™ Protein A magnetic beads, BcMag™ Protein G magnetic beads, BcMag™ Protein A/G magnetic beads, and BcMag™ Protein L magnetic beads are 1 µm uniform superparamagnetic beads coated with high density ultrapure Protein A, Protein G, Protein A/G, and Protein L, respectively. Their binding specificities to different antibody types are different.

Features and Advantages

• Quick, easy and one-step high-throughput procedure without the use of columns or filters, or laborious repetitions of pipetting or centrifugation
• High binding capacity
• Ultra-low nonspecific binding due to the hydrophilic surface
• Scalable - easy to adjust sample size and automation
• Lower cost

Applications

The BcMag™ beads can be used in cell sorting and immunoprecipitation, and for the purification of antibodies from serum samples, ascites, plasma or tissue culture supernatants.

Product Range

Amerigo Scientific offers BcMag™ Rapid IgG purification kits consisting of BcMag™ magnetic beads, binding/washing buffer, elution buffer, and neutralization buffer.

EcoMag™ Antibody Purification Particles and Kits

EcoMag™ Protein A magnetic particles, EcoMag™ Protein G magnetic particles, EcoMag™ Protein A/G magnetic particles, and EcoMag™ Protein L magnetic particles are 1-10 µm (80% of which 1µm) silica-based superparamagnetic particles covalently conjugated with Protein A, Protein G, Protein A/G, and Protein L on the surface, respectively. The particles are specifically designed, tested and quality controlled for quick and efficient one-step purification of IgG of several species from serum samples, ascites, plasma or tissue culture supernatants.

Features and Advantages

• Quick, easy and one-step high-throughput procedure, without the use of columns or filters, or laborious repetitions of pipetting or centrifugation
• High binding capacity
• Ultra-low nonspecific binding due to the hydrophilic surface
• Scalable - easy to adjust sample size and automation
• Low cost: up to half the price of competitive magnetic beads

Product Range

Amerigo Scientific offers EcoMag™ Rapid IgG purification kits consisting of EcoMag™ magnetic particles, binding/washing buffer, elution buffer, and neutralization buffer.

EcoMag™ Quick IgG Purification Kit

EcoMag™ Quick IgG Purification Kit is specifically designed for use in quick and efficient one-step purification of IgG from whole serum from various animal species with EcoMag™ superparamagnetic particles. The mild elution conditions of this product result in high protein recovery with excellent reservation of antibody activity. With this kit, 85-90% pure IgG can be obtained and dialysis is unnecessary after purification.

• Suitable for IgG purification from various animal species
• Efficient alternative to ammonium sulfate precipitation
• High degree of purity: ~ 90%