BcMag™ Tosyl-Activated Magnetic Beads

BcMag™ Tosyl-Activated Magnetic Beads are uniform, silica-based superparamagnetic beads coated with high density tolyl functional groups on the surface. The beads are used to covalently conjugate primary amine-containing ligands via a stable amide bond. BcMag™ Tosyl-Activated Magnetic Beads are most suitable for conjugation of large proteins. BcMag™ Long-arm Tosyl-Activated Magnetic Beads are recommended for conjugation of small peptides because the long- arm hydrophilic linker may reduce steric hindrance.

• Pre-activated and ready-to-use
• Recommended coupling conditions: pH 7.5–9.5 at 37°C with high efficiency
• No charge remain on the surface after coupling
• Stable covalent bond with minimal ligand leakage
• Produces reusable immunoaffinity matrices
• Low nonspecific binding
• Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads
• Applications: Cell sorting, Immunoprecipitation; Purification for Antibodies, Proteins/Peptides, DNA/RNA

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