Amerigo Scientific offers four series of ion exchange (IEX) chromatography resins of different matrix type and pore size to satisfy a variety of applications. These products are complementary with each other and are solutions of all process steps (capture, intermediate purification, and polishing) in chromatography for purifications of a variety of substances from small peptides to large proteins and viral particles.

Uni Series IEX Resins

UniCM, UniSP, UniQ, UniDEAE, and UniMSP are designed for the IEX purification of small proteins, peptides, oligo nucleotides, and small molecule such as antibiotics. Their supports are highly crosslinked polymethacrylate beads of uniformed particle size. Uni IEX resins have good hydrophilicity and low non-specific adsorbability by surface modification. Their smaller pore size makes them more suitable for IEX purification of small biomolecules. All of these resins meet the reproducibility, extensibility, and reliability requirements of today's industrial biological processes.

Characteristics

Product Name	UniCM-30S	UniCM-50XS	UniSP-30S	UniSP- 50XS	UniQ-30S	UniQ-50XS	UniDEAE-30S	UniDEAE-50XS	UniMSP-30XS	UniMSP-50XS
Support Matrix	Monodisperse Highly Crosslinked Polymethacrylate Bead
Particle Size (µm)	36	55	36	55	36	55	36	55	33	52
Pore Size (Å)	500	300	500	300	500	300	500	300	300	300
IEX Type	Weak Cation		Strong Cation		Strong Anion		Weak Anion		Strong Cation /HIC Mixed Mode
Charged Group	-CH2COO-		-(CH2)3SO3-		-CH2N+(CH3)3		-CH2CH2N(CH2CH3)2		-(CH2)3SO3-
Total Ionic Capacity (mmol/ml)	~0.23	~0.23	~0.23	~0.25	~0.23	~0.25	~0.20	~0.20	~0.25	~0.20
Dynamic Binding Capacity	~60 mg/ml (Lys)	~55 mg/ml (Lys)	~60 mg/ml (Lys)	~55 mg/ml (Lys)	/	/	/	/	~70 mg/ml (Lys)	~65 mg/ml (Lys)
Operating Pressure	< 0.8 MPa	< 0.5 MPa	< 0.8 MPa	< 0.5 MPa	< 0.8 MPa	< 0.5 MPa	< 0.8 MPa	< 0.5 MPa	< 0.8 MPa	< 0.5 MPa
pH Range	2 - 12		2 - 12		2 - 12		2 - 12		2-12

Products

Product Name	Cat. No.	Particle Diameter	Pore Size	Size
UniDEAE-30S	04023-030050	30 μm	500 Å	30ml; 100ml; 1L; 10L; 100L
UniDEAE-50XS	04023-050030	50 μm	300 Å	30ml; 100ml; 1L; 10L; 100L
UniQ-30S	04024-030050	30 μm	500 Å	30ml; 100ml; 1L; 10L; 100L
UniQ-50XS	04024-050030	50 μm	300 Å	30ml; 100ml; 1L; 10L; 100L
UniQ-50S	04024-05030	50 μm	500 Å	30ml; 100ml; 1L; 10L; 100L
UniSP-30S	04022-050030	30 μm	500 Å	30ml; 100ml; 1L; 10L; 100L
UniCM-30S	04021-030050	30 μm	500 Å	30ml; 100ml; 1L; 10L; 100L
UniCM-50XS	04021-050030	50 μm	300 Å	30ml; 100ml; 1L; 10L; 100L
UniMSP-30XS	04012-030030	30 μm	300 Å	30ml; 100ml; 1L; 10L; 100L
UniMSP-50XS	04012-050030	50 μm	300 Å	30ml; 100ml; 1L; 10L; 100L

UniGel Series of IEX Resins

UniGel series of IEX resins are divided into four categories: weak cation (CM), strong cation (SP), weak anion (DEAE), and strong anion (Q). Their supports are highly crosslinked polymethacrylate beads with uniformed particle size and open pore structure. Through polyhydroxyl surface modification and special surface extender chemistry, these UniGel IEX resins have excellent hydrophilicity and minimal nonspecific adsorption properties. They are designed to meet the bioprocessing needs of biomolecules of various sizes from small proteins to large viral particles.

Characteristics

Media Type	UniGel-CM	UniGel-SP	UniGel-DEAE	UniGel-Q
Support Matrix	Monodisperse highly crosslinked polymethacrylate beads
Particle Size (µm)	30, 80	30, 80	30, 80	30, 80
Ion Exchange Type	Weak Cation	Strong Cation	Weak Anion	Strong Anion
Charged Group	-CH2COO-	-(CH2)3SO3-	-(CH2)2N(CH2CH3)2	-CH2N+(CH3)3
Total Ionic Capacity	~0.28 mmol/ml	~0.11 mmol/ml	~0.09 mmol/ml	~0.09 mmol/ml
Dynamic Binding Capacity	~100 mg/ml (hlgG)	~115 mg/ml (Lys)	~90 mg/ml (BSA)	~90 mg/ml (BSA)
Operating Pressure	<1.0 MPa for 30 μm particle size; <0.5 MPa for 80 μm particle size
CIP pH Range	1 to 14
Working pH Range	2 to 13
Operating Temperature	4 to 30 °C
Chemical Stability	pH stability range 2-13; can be regenerated with acid or base; compatible with organic solvents; do not expose to stronger oxidizers.

Products

Product Name	Cat. No.	Particle Diameter	Pore Size	Size
UniGel®-30Q	04084-030100	30 μm	1000 Å	30ml; 100ml; 1L; 10L; 100L
UniGel®-80Q	04084-080100	80 μm	1000 Å	30ml; 100ml; 1L; 10L; 100L
UniGel®-30SP	04082-030100	30 μm	1000 Å	30ml; 100ml; 1L; 10L; 100L
UniGel®-80SP	04082-080100	80 μm	1000 Å	30ml; 100ml; 1L; 10L; 100L
UniGel®-30CM	04081-030100	30 μm	1000 Å	30ml; 100ml; 1L; 10L; 100L
UniGel®-80CM	04081-080100	80 μm	1000 Å	30ml; 100ml; 1L; 10L; 100L
UniGel®-30DEAE	04083-030100	30 μm	1000 Å	30ml; 100ml; 1L; 10L; 100L
UniGel®-80DEAE	04083-080100	80 μm	1000 Å	30ml; 100ml; 1L; 10L; 100L
NanoSP-15L	04042-015100	15 μm	1000 Å	30ml; 100ml; 1L; 10L; 100L
NanoSP-30L	04042-030100	30 μm	1000 Å	30ml; 100ml; 1L; 10L; 100L
NanoQ-15L	04044-015100	15 μm	1000 Å	30ml; 100ml; 1L; 10L; 100L
NanoQ-30L	04044-030100	30 μm	1000 Å	30ml; 100ml; 1L; 10L; 100L
NanoGel-50SP	04062-050200	50 μm	1500 Å	30ml; 100ml; 1L; 10L; 100L
NanoGel-50Q	04064-050200	50 μm	1500 Å	30ml; 100ml; 1L; 10L; 100L

Nano Series IEX Resins

NanoSP and NanoQ are, respectively, strong cation (CEX) and strong anion exchange (AEX) chromatography resins through precision monodisperse microsphere technology. These resins are rigid poly(styrene- divinylbenzene) (PS-DVB) beads with uniformed particle size and open pore structure. Through polyhydroxy surface modification, these ion exchange resins have low non-specific binding property.

NanoSP and NanoQ resins are mainly applied for high resolution polishing or intermediate purification of small or medium size biomolecules, such as peptides, nucleotides, and proteins.

Characteristics

Media Type	NanoSP-15L	NanoSP-15L	NanoQ-15L	NanoQ-15L
Support Matrix	Monodisperse PS-DVB Bead
Particle Size (µm)	15	30	15	30
lon Exchange Type	Strong Cation	Strong Cation	Strong Anion	Strong Anion
Charged Group	-(CH2)3SO3-	-(CH2)3SO3-	-(CH2N)+(CH3)2	-(CH2N)+(CH3)2
Total Ionic Capacity (µmol/ml)	0.26	0.19	0.27	0.21
Dynamic Binding Capacity (mg/ml)	80	60	55	45
Operating Pressure (MPa)	≤ 6MPa	≤ 2 MPa	≤ 6 MPa	≤ 2 MPa
CIP pH Range	1 to 14
Working pH Range	2 to 13
Operating Temperature	4 to 30 °C
Chemical Stability	pH stability range 2-13; can be regenerated with acid or base; compatible with organic solvents: do not expose to stronger oxidizers.

Products

Product Name	Cat. No.	Particle Diameter	Pore Size	Size
NanoSP-15L	04042-015100	15 μm	1000 Å	30ml; 100ml; 1L; 10L; 100L
NanoSP-30L	04042-030100	30 μm	1000 Å	30ml; 100ml; 1L; 10L; 100L
NanoQ-15L	04044-015100	15 μm	1000 Å	30ml; 100ml; 1L; 10L; 100L
NanoQ-30L	04044-030100	30 μm	1000 Å	30ml; 100ml; 1L; 10L; 100L

NanoGel - New Generation of Ion Exchange Chromatography Resins

NanoGel-50SP and NanoGel-50Q are strong cation (CEX) and strong anion exchange (AEX) chromatography resins prepared by precision monodisperse microsphere technology, respectively. These resins consist of rigid poly(styrene-divinylbenzene) (PS-DVB) beads with uniformed particle size and large open pore structure, so they maintain high performance in both bind-elute and flow-through modes. Through surface modification, the NanoGel IEX resins have low non-specific binding property. In addition, their 50 µm particle size and pore structure provides high resolution and high mass transfer for unprecedented impurity clearance independent of scale and flow rate.

NanoGel 50Sp and NanoGel 50Q are especially suitable for the purification of large biomolecules such as antibodies. NanoGel resins have high throughput and high-capacity properties, making them the optimal choice for fast and efficient processing of large amounts of protein.

Characteristics

Characteristics	NanoGel-50SP	NanoGel-50Q
Support Matrix	Monodisperse Poly(styrene-divinylbenzene) Bead
Particle Size	50 µm	50 µm
Ion Exchange Type	Strong Cation	Strong Anion
Charged Group	-CH2CH2CH2SO3	-N+(CH3)3
Total Ionic Capacity	~0.18 mmol/ml	~0.20 mmol/ml
Dynamic Binding Capacity	>100mg/ml (hIgG)	>100mg/ml (BSA)
Operating Pressure	< 20 bar (2 MPa)	< 20 bar (2 MPa）
pH Range	1 to 14	1 to 14
Operating Temperature	4 to 30 °C	4 to 30 °C

Products

Product Name	Cat. No.	Particle Diameter	Pore Size	Size
NanoGel-50SP	04062-050200	50 μm	1500 Å	30ml; 100ml; 1L; 10L; 100L
NanoGel-50Q	04064-050200	50 μm	1500 Å	30ml; 100ml; 1L; 10L; 100L