Amerigo Scientific offers four series of ion exchange (IEX) chromatography resins of different matrix type and pore size to satisfy a variety of applications. These products are complementary with each other and are solutions of all process steps (capture, intermediate purification, and polishing) in chromatography for purifications of a variety of substances from small peptides to large proteins and viral particles.

Uni Series IEX Resins

UniCM, UniSP, UniQ, UniDEAE, and UniMSP are designed for the IEX purification of small proteins, peptides, oligo nucleotides, and small molecule such as antibiotics. Their supports are highly crosslinked polymethacrylate beads of uniformed particle size. Uni IEX resins have good hydrophilicity and low non-specific adsorbability by surface modification. Their smaller pore size makes them more suitable for IEX purification of small biomolecules. All of these resins meet the reproducibility, extensibility, and reliability requirements of today's industrial biological processes.

  • Characteristics
Product Name UniCM-30S UniCM-50XS UniSP-30S UniSP- 50XS UniQ-30S UniQ-50XS UniDEAE-30S UniDEAE-50XS UniMSP-30XS UniMSP-50XS
Support Matrix Monodisperse Highly Crosslinked Polymethacrylate Bead
Particle Size (µm) 36 55 36 55 36 55 36 55 33 52
Pore Size (Å) 500 300 500 300 500 300 500 300 300 300
IEX Type Weak Cation Strong Cation Strong Anion Weak Anion Strong Cation /HIC
Mixed Mode
Charged Group -CH2COO- -(CH2)3SO3- -CH2N+(CH3)3 -CH2CH2N(CH2CH3)2 -(CH2)3SO3-
Total Ionic Capacity (mmol/ml) ~0.23 ~0.23 ~0.23 ~0.25 ~0.23 ~0.25 ~0.20 ~0.20 ~0.25 ~0.20
Dynamic Binding Capacity ~60 mg/ml (Lys) ~55 mg/ml (Lys) ~60 mg/ml (Lys) ~55 mg/ml (Lys) / / / / ~70 mg/ml (Lys) ~65 mg/ml (Lys)
Operating Pressure < 0.8 MPa < 0.5 MPa < 0.8 MPa < 0.5 MPa < 0.8 MPa < 0.5 MPa < 0.8 MPa < 0.5 MPa < 0.8 MPa < 0.5 MPa
pH Range 2 - 12 2 - 12 2 - 12 2 - 12 2-12

  • Products
Product Name Cat. No. Particle Diameter Pore Size Size
UniDEAE-30S 04023-030050 30 μm 500 Å 30ml; 100ml; 1L; 10L; 100L
UniDEAE-50XS 04023-050030 50 μm 300 Å 30ml; 100ml; 1L; 10L; 100L
UniQ-30S 04024-030050 30 μm 500 Å 30ml; 100ml; 1L; 10L; 100L
UniQ-50XS 04024-050030 50 μm 300 Å 30ml; 100ml; 1L; 10L; 100L
UniQ-50S 04024-05030 50 μm 500 Å 30ml; 100ml; 1L; 10L; 100L
UniSP-30S 04022-050030 30 μm 500 Å 30ml; 100ml; 1L; 10L; 100L
UniCM-30S 04021-030050 30 μm 500 Å 30ml; 100ml; 1L; 10L; 100L
UniCM-50XS 04021-050030 50 μm 300 Å 30ml; 100ml; 1L; 10L; 100L
UniMSP-30XS 04012-030030 30 μm 300 Å 30ml; 100ml; 1L; 10L; 100L
UniMSP-50XS 04012-050030 50 μm 300 Å 30ml; 100ml; 1L; 10L; 100L

UniGel Series of IEX Resins

UniGel series of IEX resins are divided into four categories: weak cation (CM), strong cation (SP), weak anion (DEAE), and strong anion (Q). Their supports are highly crosslinked polymethacrylate beads with uniformed particle size and open pore structure. Through polyhydroxyl surface modification and special surface extender chemistry, these UniGel IEX resins have excellent hydrophilicity and minimal nonspecific adsorption properties. They are designed to meet the bioprocessing needs of biomolecules of various sizes from small proteins to large viral particles.

  • Characteristics
Media Type UniGel-CM UniGel-SP UniGel-DEAE UniGel-Q
Support Matrix Monodisperse highly crosslinked polymethacrylate beads
Particle Size (µm) 30, 80 30, 80 30, 80 30, 80
Ion Exchange Type Weak Cation Strong Cation Weak Anion Strong Anion
Charged Group -CH2COO- -(CH2)3SO3- -(CH2)2N(CH2CH3)2 -CH2N+(CH3)3
Total Ionic Capacity ~0.28 mmol/ml ~0.11 mmol/ml ~0.09 mmol/ml ~0.09 mmol/ml
Dynamic Binding Capacity ~100 mg/ml (hlgG) ~115 mg/ml (Lys) ~90 mg/ml (BSA) ~90 mg/ml (BSA)
Operating Pressure <1.0 MPa for 30 μm particle size; <0.5 MPa for 80 μm particle size
CIP pH Range 1 to 14
Working pH Range 2 to 13
Operating Temperature 4 to 30 °C
Chemical Stability pH stability range 2-13; can be regenerated with acid or base; compatible with organic solvents; do not expose to stronger oxidizers.

  • Products
Product Name Cat. No. Particle Diameter Pore Size Size
UniGel®-30Q 04084-030100 30 μm 1000 Å 30ml; 100ml; 1L; 10L; 100L
UniGel®-80Q 04084-080100 80 μm 1000 Å 30ml; 100ml; 1L; 10L; 100L
UniGel®-30SP 04082-030100 30 μm 1000 Å 30ml; 100ml; 1L; 10L; 100L
UniGel®-80SP 04082-080100 80 μm 1000 Å 30ml; 100ml; 1L; 10L; 100L
UniGel®-30CM 04081-030100 30 μm 1000 Å 30ml; 100ml; 1L; 10L; 100L
UniGel®-80CM 04081-080100 80 μm 1000 Å 30ml; 100ml; 1L; 10L; 100L
UniGel®-30DEAE 04083-030100 30 μm 1000 Å 30ml; 100ml; 1L; 10L; 100L
UniGel®-80DEAE 04083-080100 80 μm 1000 Å 30ml; 100ml; 1L; 10L; 100L
NanoSP-15L 04042-015100 15 μm 1000 Å 30ml; 100ml; 1L; 10L; 100L
NanoSP-30L 04042-030100 30 μm 1000 Å 30ml; 100ml; 1L; 10L; 100L
NanoQ-15L 04044-015100 15 μm 1000 Å 30ml; 100ml; 1L; 10L; 100L
NanoQ-30L 04044-030100 30 μm 1000 Å 30ml; 100ml; 1L; 10L; 100L
NanoGel-50SP 04062-050200 50 μm 1500 Å 30ml; 100ml; 1L; 10L; 100L
NanoGel-50Q 04064-050200 50 μm 1500 Å 30ml; 100ml; 1L; 10L; 100L

Nano Series IEX Resins

NanoSP and NanoQ are, respectively, strong cation (CEX) and strong anion exchange (AEX) chromatography resins through precision monodisperse microsphere technology. These resins are rigid poly(styrene- divinylbenzene) (PS-DVB) beads with uniformed particle size and open pore structure. Through polyhydroxy surface modification, these ion exchange resins have low non-specific binding property.

NanoSP and NanoQ resins are mainly applied for high resolution polishing or intermediate purification of small or medium size biomolecules, such as peptides, nucleotides, and proteins.

  • Characteristics
Media Type NanoSP-15L NanoSP-15L NanoQ-15L NanoQ-15L
Support Matrix Monodisperse PS-DVB Bead
Particle Size (µm) 15 30 15 30
lon Exchange Type Strong Cation Strong Cation Strong Anion Strong Anion
Charged Group -(CH2)3SO3- -(CH2)3SO3- -(CH2N)+(CH3)2 -(CH2N)+(CH3)2
Total Ionic Capacity (µmol/ml) 0.26 0.19 0.27 0.21
Dynamic Binding Capacity (mg/ml) 80 60 55 45
Operating Pressure (MPa) ≤ 6MPa ≤ 2 MPa ≤ 6 MPa ≤ 2 MPa
CIP pH Range 1 to 14
Working pH Range 2 to 13
Operating Temperature 4 to 30 °C
Chemical Stability pH stability range 2-13; can be regenerated with acid or base; compatible with organic solvents: do not expose to stronger oxidizers.

  • Products
Product Name Cat. No. Particle Diameter Pore Size Size
NanoSP-15L 04042-015100 15 μm 1000 Å 30ml; 100ml; 1L; 10L; 100L
NanoSP-30L 04042-030100 30 μm 1000 Å 30ml; 100ml; 1L; 10L; 100L
NanoQ-15L 04044-015100 15 μm 1000 Å 30ml; 100ml; 1L; 10L; 100L
NanoQ-30L 04044-030100 30 μm 1000 Å 30ml; 100ml; 1L; 10L; 100L

NanoGel - New Generation of Ion Exchange Chromatography Resins

NanoGel-50SP and NanoGel-50Q are strong cation (CEX) and strong anion exchange (AEX) chromatography resins prepared by precision monodisperse microsphere technology, respectively. These resins consist of rigid poly(styrene-divinylbenzene) (PS-DVB) beads with uniformed particle size and large open pore structure, so they maintain high performance in both bind-elute and flow-through modes. Through surface modification, the NanoGel IEX resins have low non-specific binding property. In addition, their 50 µm particle size and pore structure provides high resolution and high mass transfer for unprecedented impurity clearance independent of scale and flow rate.

NanoGel 50Sp and NanoGel 50Q are especially suitable for the purification of large biomolecules such as antibodies. NanoGel resins have high throughput and high-capacity properties, making them the optimal choice for fast and efficient processing of large amounts of protein.

  • Characteristics
Characteristics NanoGel-50SP NanoGel-50Q
Support Matrix Monodisperse Poly(styrene-divinylbenzene) Bead
Particle Size 50 µm 50 µm
Ion Exchange Type Strong Cation Strong Anion
Charged Group -CH2CH2CH2SO3 -N+(CH3)3
Total Ionic Capacity ~0.18 mmol/ml ~0.20 mmol/ml
Dynamic Binding Capacity >100mg/ml (hIgG) >100mg/ml (BSA)
Operating Pressure < 20 bar (2 MPa) < 20 bar (2 MPa)
pH Range 1 to 14 1 to 14
Operating Temperature 4 to 30 °C 4 to 30 °C

  • Products
Product Name Cat. No. Particle Diameter Pore Size Size
NanoGel-50SP 04062-050200 50 μm 1500 Å 30ml; 100ml; 1L; 10L; 100L
NanoGel-50Q 04064-050200 50 μm 1500 Å 30ml; 100ml; 1L; 10L; 100L

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