Cell Thawing Instruments

Cryopreservation allows long-term storage and stockpiling of vital cell lines for research applications. However, freezing exposes cells to mechanical and osmotic stresses that impair cell viability and function. Proper thawing and recovery are crucial to restore cells to a healthy state. Specialized cell thawing devices provide a controlled and optimized thawing process to maximize cell viability and performance after long-term cryostorage.

The cryopreservation of cells and tissue greatly enables scientific discovery, stem cell research, and regenerative medicine by allowing the long-term preservation of precious cell samples. However, manual thawing via simple water or dry bath methods carries risks of contamination and temperature fluctuations that damage cells. Specialized cell thawing devices overcome these challenges through sterile and precisely controlled thawing to unlock the full potential of biobanking. Cell thawers provide faster thawing, more precise temperature control, no contamination, and higher cell viability than manual methods. In addition, the enclosed design protects valuable cell lines.

A variety of specialized cell thawers have been developed, based on different principles. Water bath cell thawers submerge cryovials in temperature-controlled water. Heat conduction gradually warms the samples. Water circulation and precise temperature control can prevent overheating. Closed and self-contained water bath thawers can overcome the risk of contamination. Dry bath cell thawers directly heat cryovial walls via heat conduction from the heated aluminum block or emission of infrared radiation (IR) energy. Dry units avoid cross-contamination risks and water ingress. Microwave cell thawers thaw frozen samples by microwave energy and have been used for thawing solid tissues, and large quantities of frozen cells, as well as for rapid sample processing. One of the characteristics of microwave thawing is the fast-thawing speed, which can meet high throughput requirements, but cells need to be diluted immediately after thawing to avoid damage caused by localized hot spots.

Specialized cell thawing devices provide precise temperature control and a rapid thawing process to ensure the viability and functional integrity of frozen cell samples. Amerigo Scientific offers advanced cell thawing devices to help customers ensure the availability of valuable biological resources.

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