UniSil® is a series of monodisperse spherical silica gel designed as matrix for high performance liquid chromatography (HPLC) analysis and purification. UniSil® products are developed by unique patented technology, with uniform particle size, perfect spherical shape, excellent mechanical strength and other characteristics. In addition, based on advanced microsphere precision manufacturing technology, UniSil® silica media have excellent batch-to-batch consistency, as well as tailored pore size and particle size to meet the requirements of a variety of applications. These characteristics give UniSil® silica media advantages including high column efficiency, strong column packaging and repackaging, quality consistency, and more. UniSil® silica media have been widely used for laboratory- and industrial-scale separation of synthetic compounds, natural products, and many biomolecules.
Figure 1. Representative SEM micrographs of UniSil® media with different particle sizes, showing uniform sizes and perfectly spherical shapes
- Superior mechanic strength
- Broadened pH range: 1-12
- Leading end-capping technology that improves selectivity and retention
- Good lot-to-lot stability
- Extremely low metal impurity level, greatly reducing peak tailing factor
- Ultrapure monodisperse porous spheres (CV < 5%)
- Full specifications and narrow distribution pore sizes
- High column efficiency: theoretical plate number (5 µm) > 90,000
UniSil® Product Range
Benefits of UniSil® Products
- High column efficiency and resolution, and low back pressure
- Robust column packing and repacking, and long lifetime
- High loading capacity and high selectivity
- Acid and alkaline resistance, suitable for a wide range of pH
- Consistent product quality and short delivery cycle
Highlights of UniSil® Products
Uniform particle size and perfectly spherical shape
Based on unique patented technologies, each specification of UniSil® silica media has precise particle size, high uniformity, and perfect spherical shape. Columns packed with UniSil® media have high column efficiency and low risk of frit plugging compared to the columns packed with conventional silica gel chromatography media.
Figure 3. Particle size distribution of UniSil® 10-100 C8 and analogues from other brands
Well-tailored pore structure
The mechanical strength, separation ability, and loading capacity of silica gel are affected by the pore structure. Because of their precisely controlled particle size and size distribution, as well as tailored pore size and pore volume, UniSil® media can provide optimal performance in a variety of chromatographic applications.
Figure 4. Representative SEM images of UniSil® silica media with different pore sizes
High quality surface bonding
Bonded phase UniSil® products (C18, C8, C4, Phenyl, NH2, CN, Diol, etc.) are manufactured by advanced surface bonding and end-capping technologies with high selectivity and peak symmetry. In the figure below, the sharper and more symmetrical peaks of the UniSil®
column show the high-quality bonding and end-capping in the UniSil® products.
Figure 5. Chromatographic separation of model compounds by UniSil® 5-120 C18 column and competitors from other brands
Large scale production capability and lot-to-lot consistency
Advanced manufacturing facilities and stringent quality control enable UniSil® media achieve excellent batch-to-batch consistency and scalability, meeting the high reproducibility and reliability requirements of industrial separation.
Figure 6. Particle size and BET Characterization run chart of 14 continuous batches of UniSil® 10-120
Figure 2. Comparison of 
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