BcMag™ Aldehyde-terminated Magnetic Beads Conjugation Buffer Kit

BcMag™ Amine-Terminated Magnetic Beads are uniform, silica-based superparamagnetic beads coated with high density primary Amine functional groups on the surface. The beads are used to covalently conjugate primary amine or carboxy- containing ligands. BcMag™ Amine-Terminated Magnetic Beads are most suitable for conjugation of large proteins. BcMag™ Long-arm Amine-terminated Magnetic Beads are recommended for conjugation of small peptides because the long- arm (18-atom) linker may reduce steric hindrance.

• Covalent coupling with high efficiency
• Recommended coupling conditions: pH 4.5–6, 25°C, 2–16 h
• Water-insoluble carboxy containing-ligands can be conjugated in coupling buffer containing final concentration of up to 50% purified dioxane or ethylene glycol.
• Stable covalent bond with low level of ligand leakage
• Produces reusable immunoaffinity matrices
• Low nonspecific binding
• Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads
• Applications: Purification for Antibodies, Proteins/Peptides, DNA/RNA; Cell sorting, Immunoprecipitation

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