Immobilized Metal Ion Affinity Chromatography Resins

Products List

Immobilized metal ion affinity chromatography (IMAC) resins are commonly used to purify his-tagged proteins, also untagged recombinant or native proteins. The chemical affinity of the resins is based on the coordination bonds between amino groups (histidine, tryptophan, cysteine, etc.) on protein surface and transition metal ions (Ni+2, Co+2, etc.) immobilized on the resins.

IMAC resins supplied by Amerigo Scientific are made from rigid, 80 µm mono-sized polymethacrylate matrix that carries iminodiacetic acid (IDA) chelating ligand or nitrilotriacetic acid (NTA) chelating ligand. The Ni2+ charged IMAC resin products include UniIDA-80Ni and UniNTA-80Ni. The uncharged IMAC resin products include UniIDA-80L and UniNTA-80, which can chelate with varied metal ions such as Cu2+, Ni2+, Zn2+ and Co2+.

Characteristics

Characteristics	UniIDA-80L	UniNTA-80L	UniIDA-80Ni	UniNTA-80Ni
Matrix	Mono-Sized Crosslinked Polymethacrylate Resin
Chelating Ligand	IDA	NTA	IDA	NTA
Metal Ion Charged	Uncharged	Uncharged	Ni+2	Ni+2
Particle Size	~80 µm
Metal Ion Capacity	~30 μm/mL (Ni2+)	~50 μm/mL (Ni2+)	~30 μm/mL (Ni2+)	~50 μm/mL (Ni2+)
Dynamic Binding Capacity	NA	NA	> 20mg His-tagged protein	> 15mg His-tagged protein
pH Range	2-12	2-12	2-12	2-12
Maximum Pressure (MPa)	0.5	0.5	0.5	0.5

Products

Product Name	Cat. No.	Particle Diameter	Size
UniIDA-80L	04085-080100	80 μm	10ml; 30ml; 100ml; 1L
UniNTA-80L	04086-080100	80 μm	10ml; 30ml; 100ml; 1L
UniIDA-80Ni	04087-080100	80 μm	10ml; 30ml; 100ml; 1L
UniNTA-80Ni	04088-080100	80 μm	10ml; 30ml; 100ml; 1L

Highlights

Rigid, mono-sized matrix enables high flow rate operation
Tolerant of NaOH and high concentration reducing agents, simplified sample pretreatment, much improved protein activity protection
Competitive binding capacity
Minimal ligand loss, stable in cleaning, and long service life

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